Article
Could the lower frequency of CD8+CD18+CD45RO+ lymphocytes be biomarkers of human VL?
Registro en:
CLARÊNCIO, J. et al. Could the lower frequency of CD8+CD18+CD45RO+ lymphocytes be biomarkers of human VL? International Immunolgy, v.21, n.2, p. 137-144, 2008.
0953-8178
10.1093/intimm/dxn131
Autor
Clarêncio, Jorge
Oliveira, Camila Indiani de
Favali, Cecilia Beatriz Fiuza
Medina, Omar
Caldas, Arlene de Jesus Mendes
Costa, Carlos Henrique Nery
Costa, Dorcas Lamounier
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Resumen
Toward obtaining a more comprehensive understanding of factors governing activation and/or
function during visceral leishmaniasis (VL), we have compared active disease (pre-treatment) versus
post-chemotherapy immune response in VL patients by means of ex vivo staining with different cell
markers. Our results show that during active disease, the frequency of T cells positive for CD25,
CTLA-4 and CD45RO was significantly lower in VL patients compared with healthy controls, whereas
cells staining positive for Annexin V and CD95 were significantly higher. In all cases, chemotherapy
was able to restore these frequencies to normal levels. Interestingly, significant differences in the
frequency of CD18 and in the frequency of CD45RO-positive cells were observed in the CD81 T cell
subset. These two frequencies were also significantly higher in bone marrow when compared with
peripheral blood, suggesting a possible compartmentalization of certain CD81 T cell populations
during active disease. Given that CD81 T cells have been shown to play an essential role in immunity
to infection with Leishmania, our data indicate that the lower frequency of CD181 and CD45RO1
lymphocytes in the bone marrow CD81 T cell subset may be considered a biomarker of acute VL.