Article
Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae)
Registro en:
SANTOS, D. K. D. do N. et al. Evaluation of Cytotoxic, Immunomodulatory and Antibacterial Activities of Aqueous Extract from Leaves of Conocarpus Erectus Linnaeus (Combretaceae). Journal of Pharmacy and Pharmacology, v. 70, n. 8, p. 1092–1101, 2018.
2042-7158
10.1111/jphp.12930
Autor
Santos, Dayane Kelly Dias do Nascimento
Almeida, Vanessa Silva de
Araujo, Daniel Rodrigo Cavalcante de
Harand, Wolfgang
Soares, Ana Karine de Araújo
Moreira, Leyllane Rafael
Lorena, Virgínia Maria Barros de
Magalhães, Lucimeri Paulino Machado
Ximenes, Rafael Matos
Sena, Kêsia Xisto da Fonseca Ribeiro de
Melo, Cristiane Moutinho Lagos de
Napoleão, Thiago Henrique
Lima, Cláudia Sampaio de Andrade
Yara, Ricardo
Vieira, Jeymesson Raphael Cardoso
Resumen
CAPES (Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior) OBJECTIVES: This work evaluated the antibacterial activity, cytotoxicity and immunomodulatory effect on human peripheral blood mononuclear cells (PBMCs) promoted by aqueous extract from Conocarpus erectus leaves (AELCe). METHODS: The extract was characterized by thin layer chromatography and ultraperformance liquid chromatography coupled to mass spectrometry (UPLC-MS). Cytotoxicity of AELCe (6.25-50 μg/ml) was investigated using annexin V and propidium iodide. Cytokine and nitric oxide levels in PBMCs culture supernatants exposed or not to AELCe (12.5 μg/ml) were determined, and antibacterial activity was evaluated by disc diffusion and broth microdilution methods. KEY FINDINGS: AELCe contained 3',4'-OH flavonoids, phenylpropanoglycosides, saponins, polymeric proanthocyanidins and hydrolysable tannins. Moreover, 10 other compounds were identified through UPLC-MS technique. AELCe did not affect lymphocyte viability at 6.25 and 12.5 μg/ml. IL-2, IL-10, TNF-α, IFN-γ and nitric oxide was produced in higher levels by cells treated with AELCe. Proliferation and activation of CD8+ T lymphocytes were also stimulated. AELCe showed bacteriostatic activity against clinical and antibiotic-resistant strains of Staphylococcus aureus (MIC between 250 and 1000 μg/ml). CONCLUSIONS: AELCe showed a moderate bacteriostatic activity and promoted an immunomodulatory status through higher production of Th1 cytokines, nitric oxide release and T CD8+ lymphocytes stimulation. 2050-01-01
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