Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae)

dc.creatorSantos, Dayane Kelly Dias do Nascimento
dc.creatorAlmeida, Vanessa Silva de
dc.creatorAraujo, Daniel Rodrigo Cavalcante de
dc.creatorHarand, Wolfgang
dc.creatorSoares, Ana Karine de Araújo
dc.creatorMoreira, Leyllane Rafael
dc.creatorLorena, Virgínia Maria Barros de
dc.creatorMagalhães, Lucimeri Paulino Machado
dc.creatorXimenes, Rafael Matos
dc.creatorSena, Kêsia Xisto da Fonseca Ribeiro de
dc.creatorMelo, Cristiane Moutinho Lagos de
dc.creatorNapoleão, Thiago Henrique
dc.creatorLima, Cláudia Sampaio de Andrade
dc.creatorYara, Ricardo
dc.creatorVieira, Jeymesson Raphael Cardoso
dc.date2019-04-29T13:56:25Z
dc.date2019-04-29T13:56:25Z
dc.date2018
dc.date.accessioned2023-09-26T22:19:35Z
dc.date.available2023-09-26T22:19:35Z
dc.identifierSANTOS, D. K. D. do N. et al. Evaluation of Cytotoxic, Immunomodulatory and Antibacterial Activities of Aqueous Extract from Leaves of Conocarpus Erectus Linnaeus (Combretaceae). Journal of Pharmacy and Pharmacology, v. 70, n. 8, p. 1092–1101, 2018.
dc.identifier2042-7158
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32826
dc.identifier10.1111/jphp.12930
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877874
dc.descriptionCAPES (Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior)
dc.descriptionOBJECTIVES: This work evaluated the antibacterial activity, cytotoxicity and immunomodulatory effect on human peripheral blood mononuclear cells (PBMCs) promoted by aqueous extract from Conocarpus erectus leaves (AELCe). METHODS: The extract was characterized by thin layer chromatography and ultraperformance liquid chromatography coupled to mass spectrometry (UPLC-MS). Cytotoxicity of AELCe (6.25-50 μg/ml) was investigated using annexin V and propidium iodide. Cytokine and nitric oxide levels in PBMCs culture supernatants exposed or not to AELCe (12.5 μg/ml) were determined, and antibacterial activity was evaluated by disc diffusion and broth microdilution methods. KEY FINDINGS: AELCe contained 3',4'-OH flavonoids, phenylpropanoglycosides, saponins, polymeric proanthocyanidins and hydrolysable tannins. Moreover, 10 other compounds were identified through UPLC-MS technique. AELCe did not affect lymphocyte viability at 6.25 and 12.5 μg/ml. IL-2, IL-10, TNF-α, IFN-γ and nitric oxide was produced in higher levels by cells treated with AELCe. Proliferation and activation of CD8+ T lymphocytes were also stimulated. AELCe showed bacteriostatic activity against clinical and antibiotic-resistant strains of Staphylococcus aureus (MIC between 250 and 1000 μg/ml). CONCLUSIONS: AELCe showed a moderate bacteriostatic activity and promoted an immunomodulatory status through higher production of Th1 cytokines, nitric oxide release and T CD8+ lymphocytes stimulation.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectConocarpus erectus
dc.subjectLymphocyte activation
dc.subjectMangrove
dc.subjectStaphylococcus aureus
dc.subjectTh1response
dc.subjectAdulto
dc.subjectAntibacterianos / isolamento e purificação
dc.subjectAntibacterianos / farmacologia
dc.subjectAntibacterianos / toxicidade
dc.subjectSobrevivência Celular / efeitos de drogas
dc.subjectCélulas Cultivadas
dc.subjectCombretaceae / química
dc.subjectCitocinas / análise
dc.subjectHumanos
dc.subjectFatores Imunológicos / isolamento e purificação
dc.subjectFatores Imunológicos / farmacologia
dc.subjectLeucócitos Mononucleares / efeitos de drogas
dc.subjectLeucócitos Mononucleares / imunologia
dc.subjectLinfócitos / efeitos de drogas
dc.subjectLinfócitos / imunologia
dc.subjectTestes de Sensibilidade Microbiana
dc.subjectExtratos Vegetais / isolamento e purificação
dc.subjectExtratos Vegetais / Farmacologia
dc.subjectExtratos vegetais / toxicidade
dc.subjectFolhas de plantas / química
dc.subjectPseudomonas aeruginosa / efeitos de drogas
dc.subjectStaphylococcus aureus / efeitos de drogas
dc.subjectAdulto jovem
dc.titleEvaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae)
dc.typeArticle


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