Article
Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis
Registro en:
OLIVEIRA, Edward José de et al. Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg., v. 105, n. 7, p. 414-416, 2011.
0035-9203
10.1016/j.trstmh.2011.04.010
Autor
Oliveira, Edward José de
Saliba, Soraya Wilke
Andrade, Camila Filizzola de
Rabello, Ana Lucia Teles
Resumen
In this study, the direct agglutination test (DAT), using 2-mercaptoethanol (2-ME), kaolin or N-acetylcysteine (NAC) as sample diluents, was used to assay 89 samples from visceral leishmaniasis (VL) patients and 130 samples from patients with other diseases and healthy individuals. Maintaining a cut-off of 1:100, the DAT assays with 2-ME, kaolin or NAC presented sensitivities of 94.4%, 95.5% and 100% (P = 0.09) and specificities of 99.2%, 100% and 97.7% (P = 0.17), respectively. Based on these results, we suggest that NAC can be used as a replacement for 2-ME in the DAT, increasing biosafety in the diagnosis of VL. 2030-01-01