Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis

dc.creatorOliveira, Edward José de
dc.creatorSaliba, Soraya Wilke
dc.creatorAndrade, Camila Filizzola de
dc.creatorRabello, Ana Lucia Teles
dc.date2017-01-19T15:27:54Z
dc.date2017-01-19T15:27:54Z
dc.date2011
dc.date.accessioned2023-09-26T22:03:11Z
dc.date.available2023-09-26T22:03:11Z
dc.identifierOLIVEIRA, Edward José de et al. Direct agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg., v. 105, n. 7, p. 414-416, 2011.
dc.identifier0035-9203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16898
dc.identifier10.1016/j.trstmh.2011.04.010
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874501
dc.descriptionIn this study, the direct agglutination test (DAT), using 2-mercaptoethanol (2-ME), kaolin or N-acetylcysteine (NAC) as sample diluents, was used to assay 89 samples from visceral leishmaniasis (VL) patients and 130 samples from patients with other diseases and healthy individuals. Maintaining a cut-off of 1:100, the DAT assays with 2-ME, kaolin or NAC presented sensitivities of 94.4%, 95.5% and 100% (P = 0.09) and specificities of 99.2%, 100% and 97.7% (P = 0.17), respectively. Based on these results, we suggest that NAC can be used as a replacement for 2-ME in the DAT, increasing biosafety in the diagnosis of VL.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherRoyal Society of Tropical Medicine and Hygiene
dc.rightsrestricted access
dc.subjectleishmaniose visceral
dc.subjectVisceral leishmaniasis
dc.subjectSerological diagnosis
dc.subjectDAT
dc.subjectKaolin suspension
dc.subjectNAC
dc.titleDirect agglutination test (DAT): improvement of biosafety for laboratory diagnosis of visceral leishmaniasis
dc.typeArticle


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