Brasil
| Article
Enhanced activation of eosinophils in peripheral blood and implications for eosinophilic esophagitis diagnosis.
Registro en:
BOTAN, Valéria et al. Enhanced activation of eosinophils in peripheral blood and implications for eosinophilic esophagitis diagnosis. J Gastroenterol Hepatol., v. 32, n. 7, p. 1318-1327, 2017.
0815-9319
10.1111/jgh.13710
Autor
Botan, Valéria
Borges, Tatiana Karla dos Santos
Alves, Érica Alessandra Rocha
Couto, Shirley Claudino Pereira
Seidler, Heinrich Bender Kohnert
Junqueira, Maria Imaculada Muniz
Resumen
Background and Aim: Eosinophils are markers of the eosinophilic esophagitis (EoE) disease, and this work aimed to assess whether activation of eosinophils could be a noninvasive test to contribute for EoE diagnosis.
Methods: The activation state of peripheral blood eosinophils in EoE patients and control subjects was assessed based on the morphological aspects of the eosinophil after adherence to slide. Cyclooxygenase-2 and 5-lipoxygenase expressions were evaluated by means of immunofluorescence microscopy to verify if and which eicosanoid pathway is triggered in eosinophils in blood in EoE.
Results: The eosinophils of patients with EoE were significantly more activated than those of control individuals. The lowest percentage of normal eosinophils for control subjects was 40%, while the highest percentage of eosinophils of normal aspect for patients with EoE was 32%. Considering 36% as a cutoff for normal eosinophils, this value differentiated all individuals with EoE from individuals without the disease with a sensitivity of 100%, considering the diagnosis of EoE as currently defined. Eosinophils of EoE patients showed higher expression of cyclooxygenase-2 than those of control subjects.
Conclusions: The quantification of morphological changes in eosinophils is a feasible,easy, and reliable manner to identify EoE patients. Therefore, patients with symptoms of esophageal dysfunction showing higher than 36% activated eosinophils in peripheral blood could be a useful way to help definition and diagnostic criterion for EoE. 2060-01-01