Enhanced activation of eosinophils in peripheral blood and implications for eosinophilic esophagitis diagnosis.

dc.creatorBotan, Valéria
dc.creatorBorges, Tatiana Karla dos Santos
dc.creatorAlves, Érica Alessandra Rocha
dc.creatorCouto, Shirley Claudino Pereira
dc.creatorSeidler, Heinrich Bender Kohnert
dc.creatorJunqueira, Maria Imaculada Muniz
dc.date2018-06-12T12:23:16Z
dc.date2018-06-12T12:23:16Z
dc.date2017
dc.date.accessioned2023-09-26T21:20:00Z
dc.date.available2023-09-26T21:20:00Z
dc.identifierBOTAN, Valéria et al. Enhanced activation of eosinophils in peripheral blood and implications for eosinophilic esophagitis diagnosis. J Gastroenterol Hepatol., v. 32, n. 7, p. 1318-1327, 2017.
dc.identifier0815-9319
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26815
dc.identifier10.1111/jgh.13710
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872041
dc.descriptionBackground and Aim: Eosinophils are markers of the eosinophilic esophagitis (EoE) disease, and this work aimed to assess whether activation of eosinophils could be a noninvasive test to contribute for EoE diagnosis. Methods: The activation state of peripheral blood eosinophils in EoE patients and control subjects was assessed based on the morphological aspects of the eosinophil after adherence to slide. Cyclooxygenase-2 and 5-lipoxygenase expressions were evaluated by means of immunofluorescence microscopy to verify if and which eicosanoid pathway is triggered in eosinophils in blood in EoE. Results: The eosinophils of patients with EoE were significantly more activated than those of control individuals. The lowest percentage of normal eosinophils for control subjects was 40%, while the highest percentage of eosinophils of normal aspect for patients with EoE was 32%. Considering 36% as a cutoff for normal eosinophils, this value differentiated all individuals with EoE from individuals without the disease with a sensitivity of 100%, considering the diagnosis of EoE as currently defined. Eosinophils of EoE patients showed higher expression of cyclooxygenase-2 than those of control subjects. Conclusions: The quantification of morphological changes in eosinophils is a feasible,easy, and reliable manner to identify EoE patients. Therefore, patients with symptoms of esophageal dysfunction showing higher than 36% activated eosinophils in peripheral blood could be a useful way to help definition and diagnostic criterion for EoE.
dc.description2060-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherAsian Pacific Association of Gastroenterology
dc.rightsrestricted access
dc.subjectcyclooxygenase-2
dc.subjecteicosanóides
dc.subjectesofagite eosinofílica
dc.subjecteosinófilos
dc.subjectcyclooxygenase-2
dc.subjecteicosanoids
dc.subjecteosinophilic esophagitis
dc.subjecteosinophils
dc.titleEnhanced activation of eosinophils in peripheral blood and implications for eosinophilic esophagitis diagnosis.
dc.typeArticle


Este ítem pertenece a la siguiente institución