National Council of Scientific and Technological Development (CNPq).Visceral leishmaniasis (VL) is a severe systemic and infectious disease potentially fatal when undiagnosed or untreated. So far, accurate diagnosis is still a problem, especially in endemic areas and in the tracking and screening of asymptomatic dogs, which are good reservoirs and main host in urban areas. Recombinant antigens based on DNA technology have provided more reliable serological diagnostics since the specificity can be achieved easier than using whole extracts. This work reports a sensitive piezeolectric immunosensor for anti-Leishamnia antibodies based on rLci2B recombinant antigens immobilized on quartz crystal electrode. The electrode surface was assembled by a nafion film recovered by gold nanoparticles (AuNPs) to promote a greater amount of rLci2B due to the increase of surface area and stability on bonds. The immunosensor distinguished the positive VL canine serum showing a good linearity (r = 0.989; p << 0.01) and a low relative error (<5%) at 1:400 to 1:1600 serum dilutions. The system based on AuNP achieved better results regarding sensitivity and reproducibility than the cysteamine-based immunosensor without AuNP (r = 0.769; p = 0.147). The results obtained show as a promising tool for diagnosis of the VL.