| dc.creator | Jesus, Joílson Ramos de | |
| dc.creator | Pontes-de-Carvalho, Lain Carlos | |
| dc.creator | Melo, Stella Maria Barrouin | |
| dc.creator | Neves, Neuza Maria Alcântara | |
| dc.creator | Dutra, Rosa F | |
| dc.date | 2016-08-31T17:16:02Z | |
| dc.date | 2016-08-31T17:16:02Z | |
| dc.date | 2016 | |
| dc.date.accessioned | 2023-09-26T21:04:52Z | |
| dc.date.available | 2023-09-26T21:04:52Z | |
| dc.identifier | JESUS, J. R. et al. A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum. Biochemical Engineering Journal, v. 110, p. 43–50, 2016. | |
| dc.identifier | 1369-703X | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/15563 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8868829 | |
| dc.description | National Council of Scientific and Technological Development (CNPq). | |
| dc.description | Visceral leishmaniasis (VL) is a severe systemic and infectious disease potentially fatal when undiagnosed
or untreated. So far, accurate diagnosis is still a problem, especially in endemic areas and in the tracking
and screening of asymptomatic dogs, which are good reservoirs and main host in urban areas. Recombinant
antigens based on DNA technology have provided more reliable serological diagnostics since the
specificity can be achieved easier than using whole extracts. This work reports a sensitive piezeolectric
immunosensor for anti-Leishamnia antibodies based on rLci2B recombinant antigens immobilized on
quartz crystal electrode. The electrode surface was assembled by a nafion film recovered by gold nanoparticles
(AuNPs) to promote a greater amount of rLci2B due to the increase of surface area and stability on
bonds. The immunosensor distinguished the positive VL canine serum showing a good linearity (r = 0.989;
p << 0.01) and a low relative error (<5%) at 1:400 to 1:1600 serum dilutions. The system based on AuNP
achieved better results regarding sensitivity and reproducibility than the cysteamine-based immunosensor
without AuNP (r = 0.769; p = 0.147). The results obtained show as a promising tool for diagnosis of the
VL. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Elsevier | |
| dc.rights | open access | |
| dc.subject | Immunosensor | |
| dc.subject | Quartz crystal microbalance | |
| dc.subject | Leishmania | |
| dc.subject | Leishmania infantum | |
| dc.title | A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum | |
| dc.type | Article | |
