Article
Increased sensitivity of NS1 ELISA by heat dissociation in acute dengue 4 cases
Registro en:
BUONORA, Sibelle Nogueira; et al. Increased sensitivity of NS1 ELISA by heat dissociation in acute dengue 4 cases. BMC Infectious Diseases, v.17:204, 5p, 2017.
1471-2334
10.1186/s12879-017-2306-z
Autor
Buonora, Sibelle Nogueira
Santos, Flávia Barreto dos
Daumas, Regina Paiva
Passos, Sonia Regina Lambert
Silva, Manoela Heringer da
Lima, Monique Rocha de
Nogueira, Rita Maria Ribeiro
Resumen
Dengue is an acute febrile illness considered the major arboviral disease in terms of morbidity, mortality, economic impact and dissemination worldwide. Brazil accounts for the highest notification rate, with circulation of all four dengue serotypes. The NS1 antigen is a dengue highly conserved specific soluble glycoprotein essential for viral replication and viability that can be detected 0 to 18 days from the onset of fever (peak first 3 days). It induces a strong humoral response and is known as a complement-fixing antigen. Lower NS1 test sensitivity occurs in secondary dengue infections probably due to immune complex formation impairing antigen detection by ELISA.