Article
A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
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CARLÉTTI, Dyego; et al. A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice. Clinical and Vaccine Immunology, v.20, n,8, p.1162-1169, Aug. 2013.
10.1128/CVI.00148-13
Autor
Carlétti, Dyego
Fonseca, Denise Morais da
Gembre, Ana Flavia
Masson, Ana Paula
Campos, Lívia Weijenborg
Leite, Luciana C. C.
Pires, Andréa Rodrigues
Vieira, Joseli Lannes
Silva, Célio Lopes
Bonato, Vânia Luiza Deperon
Horn, Cynthia
Resumen
Mycobacterium bovis BCG prime DNA (Mycobacterium tuberculosis genes)-booster vaccinations have been shown to induce
greater protection against tuberculosis (TB) than BCG alone. This heterologous prime-boost strategy is perhaps the most realistic
vaccination for the future of TB infection control, especially in countries where TB is endemic. Moreover, a prime-boost regimen
using biodegradable microspheres seems to be a promising immunization to stimulate a long-lasting immune response.
The alanine proline antigen (Apa) is a highly immunogenic glycoprotein secreted by M. tuberculosis. This study investigated the
immune protection of Apa DNA vaccine against intratracheal M. tuberculosis challenge in mice on the basis of a heterologous
prime-boost regimen. BALB/c mice were subcutaneously primed with BCG and intramuscularly boosted with a single dose of
plasmid carrying apa and 6,6=-trehalose dimycolate (TDM) adjuvant, coencapsulated in microspheres (BCG-APA), and were
evaluated 30 and 70 days after challenge. This prime-boost strategy (BCG-APA) resulted in a significant reduction in the bacterial
load in the lungs, thus leading to better preservation of the lung parenchyma, 70 days postinfection compared to BCG vaccinated
mice. The profound effect of this heterologous prime-boost regimen in the experimental model supports its development
as a feasible strategy for prevention of TB.