A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice

dc.creatorCarlétti, Dyego
dc.creatorFonseca, Denise Morais da
dc.creatorGembre, Ana Flavia
dc.creatorMasson, Ana Paula
dc.creatorCampos, Lívia Weijenborg
dc.creatorLeite, Luciana C. C.
dc.creatorPires, Andréa Rodrigues
dc.creatorVieira, Joseli Lannes
dc.creatorSilva, Célio Lopes
dc.creatorBonato, Vânia Luiza Deperon
dc.creatorHorn, Cynthia
dc.date2015-09-21T17:25:10Z
dc.date2015-09-21T17:25:10Z
dc.date2013
dc.date.accessioned2023-09-26T20:48:28Z
dc.date.available2023-09-26T20:48:28Z
dc.identifierCARLÉTTI, Dyego; et al. A Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice. Clinical and Vaccine Immunology, v.20, n,8, p.1162-1169, Aug. 2013.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/11676
dc.identifier10.1128/CVI.00148-13
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864246
dc.descriptionMycobacterium bovis BCG prime DNA (Mycobacterium tuberculosis genes)-booster vaccinations have been shown to induce greater protection against tuberculosis (TB) than BCG alone. This heterologous prime-boost strategy is perhaps the most realistic vaccination for the future of TB infection control, especially in countries where TB is endemic. Moreover, a prime-boost regimen using biodegradable microspheres seems to be a promising immunization to stimulate a long-lasting immune response. The alanine proline antigen (Apa) is a highly immunogenic glycoprotein secreted by M. tuberculosis. This study investigated the immune protection of Apa DNA vaccine against intratracheal M. tuberculosis challenge in mice on the basis of a heterologous prime-boost regimen. BALB/c mice were subcutaneously primed with BCG and intramuscularly boosted with a single dose of plasmid carrying apa and 6,6=-trehalose dimycolate (TDM) adjuvant, coencapsulated in microspheres (BCG-APA), and were evaluated 30 and 70 days after challenge. This prime-boost strategy (BCG-APA) resulted in a significant reduction in the bacterial load in the lungs, thus leading to better preservation of the lung parenchyma, 70 days postinfection compared to BCG vaccinated mice. The profound effect of this heterologous prime-boost regimen in the experimental model supports its development as a feasible strategy for prevention of TB.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectTuberculosis
dc.subjectDNA Vaccine
dc.subjectMycobacterium bovis
dc.subjectMycobacterium bovis BCG prime DNA
dc.subjectTuberculose
dc.subjectMycobacterium bovis
dc.subjectVacina BCG
dc.titleA Single Dose of a DNA Vaccine Encoding Apa Coencapsulated with 6,6=-Trehalose Dimycolate in Microspheres Confers Long-Term Protection against Tuberculosis in Mycobacterium bovis BCG-Primed Mice
dc.typeArticle


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