Article
An Evaluation of the Spontaneous Proliferation of Peripheral Blood Mononuclear Cells in HTLV-1-Infected Individuals Using Flow Cytometry
Registro en:
PINTO, L. A. et al. An Evaluation of the spontaneous proliferation of peripheral blood mononuclear cells in HTLV-1-infected individuals Using Flow Cytometry. ISRN Oncology, v. 2011, p. 1-6, 2011.
2090-567X
10.5402/2011/326719
Autor
Pinto, Lorena Ana
Castro Filho, Bernardo Galvão
Soares, Milena Botelho Pereira
Grassi, Maria Fernanda Rios
Resumen
The spontaneous proliferation of peripheral blood mononuclear cells (PBMCs) is a hallmark of the human T-lymphotropic virus (HTLV) type-1. Cell proliferation is usually measured using a [(3)H]thymidine incorporation assay. This study aims to quantify the spontaneous proliferation of PBMCs using flow cytometry. PBMCs were cultured for 24 to 120 hours in the presence of 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE). For comparison, PBMCs were also cultured with [(3)H]thymidine. The cutoff values for spontaneous proliferation were >0.06 for the division index and >5.8% for the percentage of divided cells. Sixty-two percent of HTLV-1-infected individuals presented spontaneous proliferation of PBMCs, which was detected in the first 24 hours. Moreover, proliferation was detected in CD4(+) and CD8(+) T-lymphocyte subsets. A positive correlation was found between the division index and [(3)H]thymidine incorporation. This method may prove useful to better understand the phenomenon of spontaneous proliferation of PBMC of patients infected with HTLV-1.