An Evaluation of the Spontaneous Proliferation of Peripheral Blood Mononuclear Cells in HTLV-1-Infected Individuals Using Flow Cytometry

dc.creatorPinto, Lorena Ana
dc.creatorCastro Filho, Bernardo Galvão
dc.creatorSoares, Milena Botelho Pereira
dc.creatorGrassi, Maria Fernanda Rios
dc.date2012-11-22T20:40:35Z
dc.date2012-11-22T20:40:35Z
dc.date2011
dc.date.accessioned2023-09-26T20:34:31Z
dc.date.available2023-09-26T20:34:31Z
dc.identifierPINTO, L. A. et al. An Evaluation of the spontaneous proliferation of peripheral blood mononuclear cells in HTLV-1-infected individuals Using Flow Cytometry. ISRN Oncology, v. 2011, p. 1-6, 2011.
dc.identifier2090-567X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5856
dc.identifier10.5402/2011/326719
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8859840
dc.descriptionThe spontaneous proliferation of peripheral blood mononuclear cells (PBMCs) is a hallmark of the human T-lymphotropic virus (HTLV) type-1. Cell proliferation is usually measured using a [(3)H]thymidine incorporation assay. This study aims to quantify the spontaneous proliferation of PBMCs using flow cytometry. PBMCs were cultured for 24 to 120 hours in the presence of 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE). For comparison, PBMCs were also cultured with [(3)H]thymidine. The cutoff values for spontaneous proliferation were >0.06 for the division index and >5.8% for the percentage of divided cells. Sixty-two percent of HTLV-1-infected individuals presented spontaneous proliferation of PBMCs, which was detected in the first 24 hours. Moreover, proliferation was detected in CD4(+) and CD8(+) T-lymphocyte subsets. A positive correlation was found between the division index and [(3)H]thymidine incorporation. This method may prove useful to better understand the phenomenon of spontaneous proliferation of PBMC of patients infected with HTLV-1.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectHTLV-1
dc.subjectProliferação celular
dc.subjectCitometria de fluxo
dc.subjectCélulas mononucleares
dc.subjectHTLV-1
dc.subjectCell proliferation
dc.subjectFlow Cytometry
dc.subjectMononuclear Cells
dc.titleAn Evaluation of the Spontaneous Proliferation of Peripheral Blood Mononuclear Cells in HTLV-1-Infected Individuals Using Flow Cytometry
dc.typeArticle


Este ítem pertenece a la siguiente institución