Article
Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina willd., a brazilian semiarid native woody plant
Registro en:
CHACON, Daisy Sotero et al. Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant. Front. Plant Sci, v. 13, n. 947558, p. 1–22, 2022.
1664-462X
10.3389/fpls.2022.947558
Autor
Chacon, Daisy Sotero
Santos, Marlon Dias Mariano
Bonilauri, Bernardo
Vilasboa, Johnatan
Costa, Cibele Tesser da
Silva, Ivanice Bezerra da
Torres, Taffarel de Melo
Araújo, Thiago Ferreira de
Roque, Alan de Araújo
Pilon, Alan Cesar
Selegatto, Denise Medeiros
Freire, Rafael Teixeira
Reginaldo, Fernanda Priscila Santos
Voigt, Eduardo Luiz
Zuanazzi, José Angelo Silveira
Scortecci, Kátia Castanho
Cavalheiro, Alberto José
Lopes, Norberto Peporine
Ferreira, Leandro De Santis
Santos, Leandro Vieira dos
Fontes, Wagner
Sousa, Marcelo Valle de
Carvalho, Paulo Costa
Fett-Neto, Arthur Germano
Giordani, Raquel Brandt
Resumen
Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh condition of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the dierentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated.
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