Article

Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts

Registro en:
PIRES, Renata de Cássia; et al. Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts. PLoS ONE, v.12, n.3, e0173922, 12p, Mar. 2017.
1932-6203
10.1371/journal.pone.0173922
Autor
Pires, Renata de Cássia
Melo, Myllena de Fátima Alheiros Dias de
Barbosa, Raquel da Hora
Roque, André Luiz Rodrigues
Institución
Resumen
The PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain segment of a conserved region of the mammalian gapdh gene.
Materias

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