Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts

dc.creatorPires, Renata de Cássia
dc.creatorMelo, Myllena de Fátima Alheiros Dias de
dc.creatorBarbosa, Raquel da Hora
dc.creatorRoque, André Luiz Rodrigues
dc.date2017-07-13T13:47:25Z
dc.date2017-07-13T13:47:25Z
dc.date2017
dc.date.accessioned2023-09-26T20:15:27Z
dc.date.available2023-09-26T20:15:27Z
dc.identifierPIRES, Renata de Cássia; et al. Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts. PLoS ONE, v.12, n.3, e0173922, 12p, Mar. 2017.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20073
dc.identifier10.1371/journal.pone.0173922
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8852633
dc.descriptionThe PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain segment of a conserved region of the mammalian gapdh gene.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectDNA de Cinetoplasto
dc.subjectLeishmania
dc.subjectReação em Cadeia da Polimerase Multiplex
dc.subjectEspécies de mamíferos selvagens
dc.subjectLeishmania sp. kDNA
dc.subjectMultiplex PCR
dc.subjectwild mammal species
dc.titleMultiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
dc.typeArticle


Este ítem pertenece a la siguiente institución