Artigo
A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis
Registro en:
Proceedings Of The National Academy Of Sciences Of The United States Of America, p. 14054–14059, 2015.
1091-6490
10.1073/pnas.1515024112
26494285
0000-0002-3034-6497
Autor
Pedrolli, Danielle Biscaro [UNESP]
Kühm, Christian
Sévin, Daniel C.
Vockenhuber, Michael P.
Sauer, Uwe
Suess, Beatrix
Mack, Matthias
Resumen
Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for biosynthesis and/or transport of riboflavin (rib genes). Cytoplasmic riboflavin is rapidly and almost completely converted to FMN by flavokinases. When cytoplasmic levels of FMN are sufficient (high levels), FMN binding to FMN riboswitches leads to a reduction of rib gene expression. We report here that the protein RibR counteracts the FMN-induced turn-off activities of both FMN riboswitches in Bacillus subtilis, allowing rib gene expression even in the presence of high levels of FMN. The reason for this secondary metabolic control by RibR is to couple sulfur metabolism with riboflavin metabolism. Department of Biotechnology, Institute for Technical Microbiology, Hochschule Mannheim, 68163 Mannheim, Germany; Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista (UNESP), 14801-902 Araraquara, Brazil Department of Biotechnology, Institute for Technical Microbiology, Hochschule Mannheim, 68163 Mannheim, Germany Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093 Zürich, Switzerland Department of Biology, Technical University Darmstadt, 64287 Darmstadt, Germany Department of Biotechnology, Institute for Technical Microbiology, Hochschule Mannheim, 68163 Mannheim, Germany Department of Biotechnology, Institute for Technical Microbiology, Hochschule Mannheim, 68163 Mannheim, Germany; Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista (UNESP), 14801-902 Araraquara, Brazil