Artigo
beta-Lactamase-based biosensor for the electrochemical determination of benzylpenicillin in milk
Registro en:
Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 210, p. 254-258, 2015.
0925-4005
10.1016/j.snb.2014.12.108
WOS:000350000000034
Autor
Prado, Thiago M. do [UNESP]
Foguel, Marcos V. [UNESP]
Goncalves, Luis Moreira
Sotomayor, Maria del Pilar T. [UNESP]
Resumen
Antibiotics are widely used in dairy cattle production, however this can pose a public health threat, particularly when antibiotic residues remain in the food products like milk. Therefore, new analytical solutions aiming the determination of antibiotics, like the still commonly used penicillin, are welcomed. This work describes a novel electrochemical biosensor, with amperometric and differential pulse voltammetric detection, based on a modified carbon paste with beta-lactamase for the determination of penicillin G, a beta-lactam antibiotic. It is a 'second-generation'biosensor since cobalt phthalocyanine was used as an electron mediator. The proposed biosensor, by differential pulse voltammetry, presented a limit of detection of 0.079 mu mol/L. Using this biosensor, a value of 43 +/- 3 mu mol/L was determined for the Michaelis-Menten enzymatic constant for penicillinase extracted from Bacillus cereus. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundacao para a Ciencia e a Tecnologia (FCT) Universidade Estadual Paulista (UNESP), Instituto de Química, Araraquara, SP, Brazil Universidade do Porto, Requimte, Departamento de Química e Bioquímica, Faculdade de Ciências, Porto, Portugal Universidade Estadual Paulista (UNESP), Instituto de Química, Araraquara, SP, Brazil FAPESP: 2011/17552-0 Fundacao para a Ciencia e a Tecnologia (FCT): SFRH/BPD/76544/2011