beta-Lactamase-based biosensor for the electrochemical determination of benzylpenicillin in milk

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade do Porto
dc.creatorPrado, Thiago M. do [UNESP]
dc.creatorFoguel, Marcos V. [UNESP]
dc.creatorGoncalves, Luis Moreira
dc.creatorSotomayor, Maria del Pilar T. [UNESP]
dc.date2015-10-22T06:17:43Z
dc.date2015-10-22T06:17:43Z
dc.date2015-04-01
dc.date.accessioned2023-09-12T06:59:56Z
dc.date.available2023-09-12T06:59:56Z
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0925400514016542
dc.identifierSensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 210, p. 254-258, 2015.
dc.identifier0925-4005
dc.identifierhttp://hdl.handle.net/11449/129621
dc.identifier10.1016/j.snb.2014.12.108
dc.identifierWOS:000350000000034
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778959
dc.descriptionAntibiotics are widely used in dairy cattle production, however this can pose a public health threat, particularly when antibiotic residues remain in the food products like milk. Therefore, new analytical solutions aiming the determination of antibiotics, like the still commonly used penicillin, are welcomed. This work describes a novel electrochemical biosensor, with amperometric and differential pulse voltammetric detection, based on a modified carbon paste with beta-lactamase for the determination of penicillin G, a beta-lactam antibiotic. It is a 'second-generation'biosensor since cobalt phthalocyanine was used as an electron mediator. The proposed biosensor, by differential pulse voltammetry, presented a limit of detection of 0.079 mu mol/L. Using this biosensor, a value of 43 +/- 3 mu mol/L was determined for the Michaelis-Menten enzymatic constant for penicillinase extracted from Bacillus cereus.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundacao para a Ciencia e a Tecnologia (FCT)
dc.descriptionUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquara, SP, Brazil
dc.descriptionUniversidade do Porto, Requimte, Departamento de Química e Bioquímica, Faculdade de Ciências, Porto, Portugal
dc.descriptionUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquara, SP, Brazil
dc.descriptionFAPESP: 2011/17552-0
dc.descriptionFundacao para a Ciencia e a Tecnologia (FCT): SFRH/BPD/76544/2011
dc.format254-258
dc.languageeng
dc.publisherElsevier B.V.
dc.relationSensors And Actuators B-chemical
dc.relation5.667
dc.relation1,406
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAmperometry
dc.subjectBenzylpenicillin
dc.subjectCarbon paste biosensor
dc.subjectDifferential pulse voltammetry
dc.subjectElectrochemistry
dc.subjectPenicillinase
dc.titlebeta-Lactamase-based biosensor for the electrochemical determination of benzylpenicillin in milk
dc.typeArtigo


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