Artigo
Effect of cryoprotectants on the reconstitution of silica nanoparticles produced by sol-gel technology
Registro en:
Journal Of Thermal Analysis And Calorimetry, v. 120, n. 1, p. 1001-1007, 2015.
1388-6150
10.1007/s10973-014-4275-4
WOS:000352139800110
Autor
Andreani, Tatiana
Kiill, Charlene Priscila [UNESP]
Souza, Ana Luiza R. de [UNESP]
Fangueiro, Joana F.
Doktorovova, Slavomira
Garcia, Maria L.
Gramiao, Maria Palmira Daflon [UNESP]
Silva, Amelia M.
Souto, Eliana B.
Resumen
Freeze-drying has widely been applied to improve the stabilization of colloidal drug carriers. In the present study, the effect of cryoprotectants on the physicochemical characteristics of silica nanoparticles (SiNP) during the freeze-drying process has been extensively investigated. SiNP were synthesized by sol-gel technology and freeze-dried in the presence of sorbitol, trehalose, and/or mannitol at different concentrations and ratios. Dynamic light scattering (DLS), atomic force microscopy (AFM), X-ray diffraction analysis (XRD), and differential scanning calorimetry (DSC) have been used for particle characterization after freeze-drying. Based on the obtained results, SiNP in the presence of mannitol showed a more crystalline behavior in comparison to nanoparticles with sorbitol or trehalose (confirmed by DSC and XRD). SiNP in the presence of trehalose showed a more crystalline structure than SiNP in the presence of sorbitol. However, trehalose was more efficient in preserving the particle size of nanoparticles during the freeze-drying process. The optimal concentration of trehalose for preserving silica nanoparticles was 10 % at a ratio of (1:1). During the freeze-drying process, trehalose is able to replace water molecules due to the strong interaction via hydrogen bounds between silanol groups present in SiNP surface and the sugar, forming a stable layer around the particle and thus preserving the particle physical properties. Fundação para a Ciência e a Tecnologia (FCT) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) European Union Funds (FEDER/COMPETE) Univ Tras Os Montes &Alto Douro UTAD, Dept Biol &Environm, P-5001801 Vila Real, Portugal UTAD, Ctr Res &Technol Agro Environm &Biol Sci CITAB, Vila Real, Portugal Univ Fernando Pessoa, Res Ctr Biomed CEBIMED, P-4249004 Oporto, Portugal UNESP Univ Estadual Paulista, Dept Pharmaceut Sci, Sao Paulo, Brazil Univ Fernando Pessoa, Fac Hlth Sci, P-4200150 Oporto, Portugal Univ Barcelona, Fac Pharm, Dept Phys Chem, E-08028 Barcelona, Spain Univ Coimbra FFUC, Fac Pharm, Polo Ciencias Saude, P-3000548 Coimbra, Portugal UNESP Univ Estadual Paulista, Dept Pharmaceut Sci, Departamento de Farmacos e Médicamentos, Sao Paulo, Brazil FCT: SFRH/BD/60640/2009 FCT: SFRH/BD/80335/2011 FCT: SFRH/BD/60552/2009 FCT: PTDC/SAU-FAR/113100/2009 FCT: PEst-C/AGR/UI4033/2011 FEDER/COMPETE: PTDC/SAU-FAR/113100/2009 FEDER/COMPETE: PEst-C/AGR/UI4033/2011 FEDER/COMPETE: FCOMP-01-0124-FEDER-022696