Artigo
Biotransformed soybean extract induces cell death of estrogen-dependent breast cancer cells by modulation of apoptotic proteins
Registro en:
Nutrition And Cancer-an International Journal, v. 67, n. 4, p. 612-619, 2015.
0163-5581
10.1080/01635581.2015.1015744
WOS:000353771000008
5772565774304020
Autor
Stocco, Bianca
Toledo, Karina A. [UNESP]
Fumagalli, Helen F.
Bianchini, Francine J.
Fortes, Vanessa S.
Fonseca, Maria Jose V.
Toloi, Maria Regina T.
Resumen
The process of soybean biotransformation increases the quantity of isoflavones (daidzein and genistein), which besides being considered an alternative to estroprogestive hormone replacement therapy (HRT), are able of hindering the growth and development of tumor cells. We investigated the effects of soybean extract biotransformed by fungus on estrogen-dependent (MCF-7) and nondependent (SK-BR-3) breast cell lines. Cells were treated with different concentrations of biotransformed (BSE) and nonbiotransformed soybean extract (SE), or daidzein (D) and genistein (G) patterns isolated and in combination (D + G). Afterwards, we analyzed cell viability by MTT assay, phosphatidylserine exposure and cell permeability by flow cytometry; expression of apoptotic proteins by Western blotting. BSE promoted reduction in cell viability and increase in DNA degradation in both cell lines. In addition, we verified increase in cell permeability and in the expression of phosphatidylserine, as well as modulation in the expression of apoptotic proteins in MCF-7 cells. The cells did not show any signs of cell death when incubated with the controls (D, G, and D + G). Unknown components found in the BSE induce cell death by apoptosis and necrosis, mainly in MCF-7 cells. These processes depend on the activation of caspase-3 and involve an increase in the expression of proapoptotic molecules. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Univ Sao Paulo, Lab Clin Cytol, Fac Pharmaceut Sci Ribeirao Preto, BR-05508 Sao Paulo, Brazil Univ Estadual Paulista UNESP FCL Assis, Dept Biol Sci, Assis, SP, Brazil Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Lab Photochemoprotect &Qual Control, BR-05508 Sao Paulo, Brazil Univ Estadual Paulista UNESP FCL Assis, Dept Biol Sci, Assis, SP, Brazil