Biotransformed soybean extract induces cell death of estrogen-dependent breast cancer cells by modulation of apoptotic proteins

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorStocco, Bianca
dc.creatorToledo, Karina A. [UNESP]
dc.creatorFumagalli, Helen F.
dc.creatorBianchini, Francine J.
dc.creatorFortes, Vanessa S.
dc.creatorFonseca, Maria Jose V.
dc.creatorToloi, Maria Regina T.
dc.date2015-10-21T20:46:49Z
dc.date2015-10-21T20:46:49Z
dc.date2015-05-19
dc.date.accessioned2023-09-12T06:52:07Z
dc.date.available2023-09-12T06:52:07Z
dc.identifierhttp://www.tandfonline.com/doi/abs/10.1080/01635581.2015.1015744#.VfrMixFViko
dc.identifierNutrition And Cancer-an International Journal, v. 67, n. 4, p. 612-619, 2015.
dc.identifier0163-5581
dc.identifierhttp://hdl.handle.net/11449/129294
dc.identifier10.1080/01635581.2015.1015744
dc.identifierWOS:000353771000008
dc.identifier5772565774304020
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778634
dc.descriptionThe process of soybean biotransformation increases the quantity of isoflavones (daidzein and genistein), which besides being considered an alternative to estroprogestive hormone replacement therapy (HRT), are able of hindering the growth and development of tumor cells. We investigated the effects of soybean extract biotransformed by fungus on estrogen-dependent (MCF-7) and nondependent (SK-BR-3) breast cell lines. Cells were treated with different concentrations of biotransformed (BSE) and nonbiotransformed soybean extract (SE), or daidzein (D) and genistein (G) patterns isolated and in combination (D + G). Afterwards, we analyzed cell viability by MTT assay, phosphatidylserine exposure and cell permeability by flow cytometry; expression of apoptotic proteins by Western blotting. BSE promoted reduction in cell viability and increase in DNA degradation in both cell lines. In addition, we verified increase in cell permeability and in the expression of phosphatidylserine, as well as modulation in the expression of apoptotic proteins in MCF-7 cells. The cells did not show any signs of cell death when incubated with the controls (D, G, and D + G). Unknown components found in the BSE induce cell death by apoptosis and necrosis, mainly in MCF-7 cells. These processes depend on the activation of caspase-3 and involve an increase in the expression of proapoptotic molecules.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniv Sao Paulo, Lab Clin Cytol, Fac Pharmaceut Sci Ribeirao Preto, BR-05508 Sao Paulo, Brazil
dc.descriptionUniv Estadual Paulista UNESP FCL Assis, Dept Biol Sci, Assis, SP, Brazil
dc.descriptionUniv Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Lab Photochemoprotect &Qual Control, BR-05508 Sao Paulo, Brazil
dc.descriptionUniv Estadual Paulista UNESP FCL Assis, Dept Biol Sci, Assis, SP, Brazil
dc.format612-619
dc.languageeng
dc.publisherRoutledge Journals, Taylor &francis Ltd
dc.relationNutrition And Cancer-an International Journal
dc.relation2.261
dc.relation0,745
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.titleBiotransformed soybean extract induces cell death of estrogen-dependent breast cancer cells by modulation of apoptotic proteins
dc.typeArtigo


Este ítem pertenece a la siguiente institución