Artigo
Apoptosis of Purkinje and Granular Cells of the Cerebellum Following Chronic Ethanol Intake
Registration in:
Cerebellum. New York: Springer, v. 13, n. 6, p. 728-738, 2014.
1473-4222
10.1007/s12311-014-0591-2
WOS:000345284700008
1739564105219382
Author
Oliveira, Suelen A. [UNESP]
Chuffa, Luiz Gustavo A. [UNESP]
Fioruci-Fontanelli, Beatriz Aparecida [UNESP]
Lizarte Neto, Fermino Sanches
Novais, Paulo Cezar
Tirapelli, Luiz Fernando
Oishi, Jorge Camargo
Takase, Luiz Fernando
Stefanini, Maira Aparecida
Martinez, Marcelo
Martinez, Francisco Eduardo [UNESP]
Abstract
Ethanol alters motricity, learning, cognition, and cellular metabolism in the cerebellum. We evaluated the effect of ethanol on apoptosis in Golgi, Purkinje, and granule cells of the cerebellum in adult rats. There were two groups of 20 rats: a control group that did not consume ethanol and an experimental group of UChA rats that consumed ethanol at 10 % (<2 g ethanol/kg body weight/day). At 120 days old, rats were anesthetized and decapitated, and their cerebella were collected and fixed. Cerebellar sections were subjected to immunohistochemistry for terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL), caspase-3, X-linked inhibitor of apoptosis protein (XIAP), and insulin-like growth factor 1-receptor (IGF-1R); real-time PCR (RT-PCR) to determine caspase-3, XIAP, and IGF-1R gene expression; and transmission electron microscopy (TEM). We identified fragmentation of DNA and an increase in caspase-3 protein and XIAP in Purkinje cells, whereas granule cells exhibited increased caspase-3 and XIAP. IGF-1R expression was unchanged. There was no significant difference in gene expression of caspase-3, XIAP, and IGF-1R. There were an increase in lipid droplets, a reduction in the cellular cytoplasm in electrondense nuclei, and changes in the myelin sheath in the cerebellar cortex. In conclusion, our data demonstrated that ethanol induced apoptosis in the Purkinje and granule cells of the cerebellum of adult UChA rats. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Univ Estadual Paulista UNESP, Inst Biosci, Grad Program Gen & Appl Biol, BR-18618970 Botucatu, SP, Brazil Univ Estadual Paulista UNESP, Dept Anat, Inst Biosci, BR-18618970 Botucatu, SP, Brazil Univ Sao Paulo, Fac Med, Dept Surg & Anat, BR-14049900 Ribeirao Preto, SP, Brazil Univ Fed Sao Carlos, Dept Physiol, BR-13565905 Sao Carlos, SP, Brazil Univ Fed Sao Carlos, Dept Morphol & Pathol, BR-13565905 Sao Carlos, SP, Brazil Univ Estadual Paulista UNESP, Inst Biosci, Grad Program Gen & Appl Biol, BR-18618970 Botucatu, SP, Brazil Univ Estadual Paulista UNESP, Dept Anat, Inst Biosci, BR-18618970 Botucatu, SP, Brazil FAPESP: 11/50466-0