Apoptosis of Purkinje and Granular Cells of the Cerebellum Following Chronic Ethanol Intake

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Federal de São Carlos (UFSCar)
dc.creatorOliveira, Suelen A. [UNESP]
dc.creatorChuffa, Luiz Gustavo A. [UNESP]
dc.creatorFioruci-Fontanelli, Beatriz Aparecida [UNESP]
dc.creatorLizarte Neto, Fermino Sanches
dc.creatorNovais, Paulo Cezar
dc.creatorTirapelli, Luiz Fernando
dc.creatorOishi, Jorge Camargo
dc.creatorTakase, Luiz Fernando
dc.creatorStefanini, Maira Aparecida
dc.creatorMartinez, Marcelo
dc.creatorMartinez, Francisco Eduardo [UNESP]
dc.date2015-03-18T15:53:10Z
dc.date2015-03-18T15:53:10Z
dc.date2014-12-01
dc.date.accessioned2023-09-09T11:03:44Z
dc.date.available2023-09-09T11:03:44Z
dc.identifierhttp://dx.doi.org/10.1007/s12311-014-0591-2
dc.identifierCerebellum. New York: Springer, v. 13, n. 6, p. 728-738, 2014.
dc.identifier1473-4222
dc.identifierhttp://hdl.handle.net/11449/116376
dc.identifier10.1007/s12311-014-0591-2
dc.identifierWOS:000345284700008
dc.identifier1739564105219382
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8765863
dc.descriptionEthanol alters motricity, learning, cognition, and cellular metabolism in the cerebellum. We evaluated the effect of ethanol on apoptosis in Golgi, Purkinje, and granule cells of the cerebellum in adult rats. There were two groups of 20 rats: a control group that did not consume ethanol and an experimental group of UChA rats that consumed ethanol at 10 % (<2 g ethanol/kg body weight/day). At 120 days old, rats were anesthetized and decapitated, and their cerebella were collected and fixed. Cerebellar sections were subjected to immunohistochemistry for terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL), caspase-3, X-linked inhibitor of apoptosis protein (XIAP), and insulin-like growth factor 1-receptor (IGF-1R); real-time PCR (RT-PCR) to determine caspase-3, XIAP, and IGF-1R gene expression; and transmission electron microscopy (TEM). We identified fragmentation of DNA and an increase in caspase-3 protein and XIAP in Purkinje cells, whereas granule cells exhibited increased caspase-3 and XIAP. IGF-1R expression was unchanged. There was no significant difference in gene expression of caspase-3, XIAP, and IGF-1R. There were an increase in lipid droplets, a reduction in the cellular cytoplasm in electrondense nuclei, and changes in the myelin sheath in the cerebellar cortex. In conclusion, our data demonstrated that ethanol induced apoptosis in the Purkinje and granule cells of the cerebellum of adult UChA rats.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniv Estadual Paulista UNESP, Inst Biosci, Grad Program Gen & Appl Biol, BR-18618970 Botucatu, SP, Brazil
dc.descriptionUniv Estadual Paulista UNESP, Dept Anat, Inst Biosci, BR-18618970 Botucatu, SP, Brazil
dc.descriptionUniv Sao Paulo, Fac Med, Dept Surg & Anat, BR-14049900 Ribeirao Preto, SP, Brazil
dc.descriptionUniv Fed Sao Carlos, Dept Physiol, BR-13565905 Sao Carlos, SP, Brazil
dc.descriptionUniv Fed Sao Carlos, Dept Morphol & Pathol, BR-13565905 Sao Carlos, SP, Brazil
dc.descriptionUniv Estadual Paulista UNESP, Inst Biosci, Grad Program Gen & Appl Biol, BR-18618970 Botucatu, SP, Brazil
dc.descriptionUniv Estadual Paulista UNESP, Dept Anat, Inst Biosci, BR-18618970 Botucatu, SP, Brazil
dc.descriptionFAPESP: 11/50466-0
dc.format728-738
dc.languageeng
dc.publisherSpringer
dc.relationCerebellum
dc.relation3.199
dc.relation1,705
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectApoptosis
dc.subjectXIAP
dc.subjectIGF-1R
dc.subjectCaspase-3
dc.subjectCerebellum
dc.subjectUChA rats
dc.titleApoptosis of Purkinje and Granular Cells of the Cerebellum Following Chronic Ethanol Intake
dc.typeArtigo


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