Artigo
Luminescent threat: toxicity of light stick attractors used in pelagic fishery
Registro en:
Scientific Reports. London: Nature Publishing Group, v. 4, 11 p., 2014.
2045-2322
10.1038/srep05359
WOS:000337499400008
WOS000337499400008.pdf
Autor
Oliveira, Tiago Franco de
Medeiros da Silva, Amanda Lucila
Moura, Rafaela Alves de
Bagattini, Raquel [UNESP]
Falcao de Oliveira, Antonio Anax
Gennari de Medeiros, Marisa Helena
Di Mascio, Paolo
Arruda Campos, Ivan Persio de [UNESP]
Barretto, Fabiano Prado
Henriques Bechara, Etelvino Jose
Melo Loureiro, Ana Paula de
Resumen
Light sticks (LS) are sources of chemiluminescence commonly used in pelagic fishery, where hundreds are discarded and reach the shores. Residents from fishing villages report an improper use of LS contents on the skin. Given the scarce information regarding LS toxicity, the effects of LS solutions in cell cultures were evaluated herein. Loss of viability, cell cycle changes and DNA fragmentation were observed in HepG2 cell line and skin fibroblasts. A non-cytotoxic LS concentration increased the occurrence of the mutagenic lesion 1,N-6-epsilon dAdo in HepG2 DNA by three-fold. Additionally, in vitro incubations of spent LS contents with DNA generated dGuo-LS adducts, whose structure elucidation revealed the presence of a reactive chlorinated product. In conclusion, the LS contents were found to be highly cyto- and genotoxic. Our data indicate an urgent need for LS waste management guidelines and for adequate information regarding toxic outcomes that may arise from human exposure. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) PRPUSP (Pro-Reitoria de Pesquisa da Universidade de Sao Paulo) NAP Redoxoma (PRPUSP) Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo, Brazil Univ Estadual Paulista, Inst Ciencias & Saude, Sao Paulo, Brazil Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo, Brazil Univ Estadual Paulista, Inst Ciencias Exatas & Tecnol, Sao Paulo, Brazil Global Garbage, Hamburg, Germany Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, Diadema, SP, Brazil Univ Estadual Paulista, Inst Ciencias & Saude, Sao Paulo, Brazil Univ Estadual Paulista, Inst Ciencias Exatas & Tecnol, Sao Paulo, Brazil FAPESP: 12/21636-8, 2012/08617-4 FAPESP: 06/56530-4 FAPESP: 06/57929-8 FAPESP: 09/54816-6 FAPESP: 12/08616-8 FAPESP: 573530/2008-4 NAP Redoxoma (PRPUSP)2011.1.9352.1.8 FAPESP: 13/07937-8