Identificação e perfil de sensibilidade de microrganismos isolados em ambiente cirúrgico de hospital veterinário

Abstract

Hospital infections are major public health problems and become even more severe when they involve multidrug-resistant pathogens. Thus, the present study aimed to evaluate environmental contamination and respective antimicrobial sensitivity in a veterinary hospital. For this, swabs were collected from five dog hospitalization (IC) boxes, five cat hospitalization (IG) boxes, five intensive care unit (UTI) boxes, and three surgical tables in the operating room from the surgical ward (BC) before and after performing a process of hospital disinfection. In addition, swabs were collected from specific sites of the surgical ward (instrument tables, surgical focus, Bain circuit, Baraka, incubator, and postoperative recovery table). The swab samples were submitted to bacterial culture, phenotypic identification of microorganisms, and evaluation of the sensitivity profile of bacteria. Multidrug-resistant (MRD) bacteria were isolated from all collection sites, with coagulase-negative staphylococci (CoNS) being the most frequent bacterium, representing 45% of the microorganisms isolated. Moreover, MRD microorganisms of nosocomial importance were isolated, such as Pseudomonas sp. and Enterobacter sp., isolated before disinfection in IG and IC, respectively, and Enterococcus sp. isolated in IG after disinfection, which demonstrates the presence of microorganisms after disinfection, but with altered isolation profile. The observed results demonstrate the importance of implementing appropriate biosecurity measures to protect animal and human health.