Lipopolysaccharide from Porphyromonas gingivalis, but Not from Porphyromonas endodontalis, Induces Macrophage M1 Profile

dc.creatorVeloso, Pablo
dc.creatorFernández, Alejandra
dc.creatorAstorga, Jessica
dc.creatorGonzález-Quintanilla, David
dc.creatorCastro, Alfredo
dc.creatorEscobar, Alejandro
dc.creatorHoare, Anilei
dc.creatorHernández, Marcela
dc.date2022-10-11T19:02:23Z
dc.date2022-10-11T19:02:23Z
dc.date2022-09-02
dc.date.accessioned2023-08-25T20:02:59Z
dc.date.available2023-08-25T20:02:59Z
dc.identifier1422-0067
dc.identifier10.3390/ijms231710011
dc.identifierhttps://hdl.handle.net/20.500.12536/1826
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8438129
dc.descriptionApical Lesions of Endodontic Origin (ALEO) are initiated by polymicrobial endodontic canal infection. Porphyromonas gingivalis (Pg) and Porphyromonas endodontalis (Pe) lipopolysaccharides (LPS) can induce a pro-inflammatory macrophage response through their recognition by TLR2 and TLR4. However, polarization responses induced by Pg and/or Pe LPS in macrophages are not fully understood. We aimed to characterize the polarization profiles of macrophages differentiated from THP-1 cells following Pg and/or Pe LPS stimulation from reference strain and clinical isolates. A modified LPS purification protocol was implemented and the electrophoretic LPS profiles were characterized. THP-1 human monocytes differentiated to macrophages were stimulated with Pg and Pe LPS. Polarization profiles were characterized through cell surface markers and secreted cytokines levels after 24 h of stimulation. TLR2 and TLR4 cell surfaces and transcriptional levels were determined after 24 or 2 h of LPS stimulation, respectively. LPS from Pg induced a predominant M1 profile in macrophages evidenced by changes in the expression of the surface marker CD64 and pro-inflammatory cytokine profiles, TNF-α, IL-1β, IL-6, and IL-12. Pe LPS was unable to induce a significant response. TLR2 and TLR4 expressions were neither modified by Pg or Pe LPS. Pg LPS, but not Pe LPS, induced a macrophage M1 Profile.
dc.formatapplication/pdf
dc.languageen
dc.sourceInternational Journal of Molecular Sciences
dc.subjectPorphyromonas gingivalis
dc.subjectPorphyromonas endodontalis
dc.subjectLipopolysaccharide
dc.titleLipopolysaccharide from Porphyromonas gingivalis, but Not from Porphyromonas endodontalis, Induces Macrophage M1 Profile
dc.typeArtículo de revista


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