Dendrobium nobile in vitro flowering induction

dc.creatorNadal, Michele Carla
dc.creatorSilva Andrade, Gracielle Vidal
dc.creatorNunes Flores, José Henrique
dc.creatordos Reis, Michele Valquíria
dc.creatorRodrigues, Vantuil Antonio
dc.creatorPasqual, Moacir
dc.date2023-07-14T13:55:23Z
dc.date2023-07-14T13:55:23Z
dc.date2023-05-22
dc.date.accessioned2023-08-25T20:02:06Z
dc.date.available2023-08-25T20:02:06Z
dc.identifier2447-536X
dc.identifier10.1590/2447-536X.v29i2.2612
dc.identifierhttps://hdl.handle.net/20.500.12536/1937
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8437806
dc.descriptionIn vitro flowering is a technique used in genetic improvement that accelerates generations and favors the faster fixation of new traits of agronomic and market interest. The study aimed to establish a protocol for the in vitro flowering of Dendrobium nobile, through the combined temperature and of the growth regulator thidiazuron (TDZ) effects. Experiments was performed in a flask that was kept in a BOD incubator at 18, 21, or 24 °C or in a growth room at 26 °C. The TDZ concentrations were 0.0, 0.5, 1.0, and 2.0 mg L-1. The highest percentage of flowering shoots of 13.7% occurred at a concentration of 2 mg L-1 of TDZ grown at a temperature of 18 °C. Dendrobium nobile in vitro flowering was promoted in plants cultivated in ½ MS supplemented with 30 g L-1 sucrose, 5.5 g L-1 agar, 100 mL L-1 coconut water, pH 6.0, and 2.0 mg L-1 TDZ at a controlled temperature of 18 °C and a photoperiod of 16 h. The in vitro flowering induction protocol of the D. nobile species could be used or improved for future studies.Keywords: flowering, temperature, thidiazuron, Orchidaceae.
dc.formatapplication/pdf
dc.languageen
dc.sourceOrnamental Horticulture
dc.subjectFlowering
dc.subjectTemperature
dc.subjectThidiazuro
dc.subjectOrchidacea
dc.titleDendrobium nobile in vitro flowering induction
dc.typeArtículo de revista


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