Articulo
Gills CYP1A of Oncorhynchus mykiss as a sensitive biomarker of crude oil pollution in freshwater environments
Environmental Toxicology and Pharmacology
Registro en:
1150934
1150934
Autor
Leggieri, Leonardo Ramón
De Anna, Julieta Soledad
Cárcamo-Matus, Juan Guillermo
Cerón, Gerardo Abel
Arias-Darraz, Luis
Panebianco, Antonella
Luquet, Carlos Marcelo
Institución
Resumen
The induction of CYP1A activity (EROD) and protein expression was compared in liver and gills of rainbow trout from a stream polluted with crude oil, and through laboratory exposures to 1% and 5% of water accommodated fraction of the crude oil (WAF) for 1 and 4 days. Gills EROD increased 1.6-2.7-fold in fish from the polluted stream and during experiments, while liver EROD was induced only by 1% WAF at day 1 (1.5-fold). Contrastingly, crude oil pollution strongly induced both liver and gills CYP1A protein expression in the field (14-36-fold) and in experiments (4-25-fold). This highlights that crude oil induced CYP1A activity markedly in gills but only slightly or not at all in the liver, suggesting that differences between organ EROD activities are related to the modulation of CYP1A enzyme activity but not to the regulation at transcriptional or translational levels.Copyright © 2019 Elsevier B.V. All rights reserved. KEYWORDS: CYP450; Petroleum pollution; Rainbow trout Regular 2015 FONDECYT FONDECYT