Articulo
Bacteriostatic anti-Vibrio parahaemolyticus activity of Pseudoalteromonas sp strains DIT09, DIT44 and DIT46 isolated from Southern Chilean intertidal Perumytilus purpuratus
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY;
Neurosci. Lett.
Registro en:
1573-0972
D06I1054
D06I1054
WOS:000304135200008
0304-3940
Autor
Aranda, Carlos P.
Valenzuela, Cristian
Barrientos, Javier
Paredes, Javier
Leal, Pablo
Maldonado, Miguel
Godoy, Felix A.
Osorio, Carlos G.
Institución
Resumen
We characterised the anti- (anti-) marine bacteria DIT09, DIT44 and DIT46 isolated from the intertidal mussel . The 16S rRNA gene sequences identify a sp. that form a clade with and . The strains produced bacteriostatic anti- agents during the exponential growth phase, which were also active against and , but not on other Gram positive and Gram negative bacteria. Bacteriostatic agents could be permeated by analytic ultra-filtration with 3.5 kDa cut-off, partially precipitated with 70 and 90 % ammonium sulphate, but not extracted with ethyl acetate. Reverse-phase HPLC revealed the production of a set of 5-6 active compounds by each strain (elution from 20 to 40 % acetonitrile), with similar but non identical HPLC patterns. Additionally, was able to progressively overcome the inhibition of antibiotics in trypticase soy agar with Fe(III) 0.5 up to 2 mM, suggesting the involvement of a set of novel siderophore or active molecules targeted at different Fe-siderophore uptake systems. The overall findings suggest that sp. DIT strains produce a putatively novel class of bacteriostatic and probably amphiphilic anti- agents, indicating the need for further studies with chemical purification followed by their structural and functional characterization. Finally, the crude cell-free extracts, as well as the strains incubated at 10(3) and 10(5) c.f.u./mL, did not cause mortality in nauplii, suggesting that these bacteria are serious candidates for further probiotic evaluations with shellfish and fish cultures. This work was supported by Chilean "Fondo de Fomento al Desarrollo Cientifico y Tecnologico" (FONDEF D06I1054). The authors thanks to Matthew Lee for his critical review of this manuscript. 7 FONDEF caranda@ulagos.cl Fondo de Fomento al Desarrollo Cientifico y Tecnologico [FONDEF D06I1054] FONDEF