Article
Characterization of phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis isolates from a city in Mexico
Fecha
2005Registro en:
10.1016/j.eimc.2014.04.005
Autor
Flores-Ramirez, N.
Elizalde-Pena, E.A.
Vasquez-García, S.R.
Gonzalez-Hernandez, J.
Martinez-Ruvalcaba, A.
Sanchez, I.C.
Luna-Barcenas, G.
Gupta, R.B.
Institución
Resumen
The synthesis, characterization and degradation of a hybrid chitosan (CTS)/glycidyl methacrylate (GMA) material are reported. These versatile materials (natural-synthetic materials) are potential candidates for dental restoratives. All materials were characterized by infrared spectroscopy (FT-IR), X-ray diffraction and thermal (DSC) analysis. Particular attention was paid to the thermal stability and chemical resistance of the hybrid CTS materials. From dynamical rheological tests, it was concluded that CTS-GMA solutions behave as physical hydrogels. These pH-sensitive gels are an example of stimuli-responsive polymers, also known as 'smart polymers'. " VSP 2005.",,,,,,"10.1163/1568562053700174",,,"http://hdl.handle.net/20.500.12104/39985","http://www.scopus.com/inward/record.url?eid=2-s2.0-24044479108&partnerID=40&md5=f86416e303dc646b152328c701f5b5fd",,,,,,"4",,"Journal of Biomaterials Science, Polymer Edition",,"473 488",,"16",,"Scopus WOS",,,,,,"Biopolymer; Chemical resistance; Chitosan; Glycidyl methacrylate; Thermal resistance",,,,,,"Characterization and degradation of functionalized chitosan with glycidyl methacrylate",,"Article"
"41779","123456789/35008",,"Flores-Treviño, S., Servicio de Gastroenterología, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo LeónMonterrey, Nuevo León, Mexico; Morfín-Otero, R., Hospital Civil de Guadalajara, Instituto de Patología Infecciosa y Experimental, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Rodríguez-Noriega, E., Hospital Civil de Guadalajara, Instituto de Patología Infecciosa y Experimental, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; González-Díaz, E., Hospital Civil de Guadalajara, Instituto de Patología Infecciosa y Experimental, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Pérez-Gómez, H.R., Hospital Civil de Guadalajara, Instituto de Patología Infecciosa y Experimental, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Mendoza-Olazarán, S., Servicio de Gastroenterología, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo LeónMonterrey, Nuevo León, Mexico; Balderas-Rentería, I., Laboratorio de Ingeniería Genética y Genómica, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo LeónMonterrey, Nuevo León, Mexico; González, G.M., Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo LeónMonterrey, Nuevo León, Mexico; Garza-González, E., Servicio de Gastroenterología, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo LeónMonterrey, Nuevo León, Mexico, Departamento de Patología Clínica, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo LeónMonterrey, Nuevo León, Mexico",,"Flores-Trevino, S. Morfin-Otero, R. Rodriguez-Noriega, E. Gonzalez-Diaz, E. Perez-Gomez, H.R. Mendoza-Olazaran, S. Balderas-Renteria, I. Gonzalez, G.M. Garza-Gonzalez, E.",,"2015",,"Introduction The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains has become a worldwide health care problem, making treatment of tuberculosis difficult. The aim of this study was to determine phenotypic resistance and gene mutations associated with MDR of clinical isolates of Mycobacterium tuberculosis from Guadalajara, Mexico. Methods One hundred and five isolates were subjected to drug susceptibility testing to first line drugs using the proportion and Mycobacteria Growth Indicator Tube (MGIT) methods. Genes associated with isoniazid (inhA, katG, ahpC) and rifampicin (rpoB) resistance were analyzed by either pyrosequencing or PCR-RFLP. Results Resistance to any drug was detected in 48.6% of isolates, of which 40% were isoniazid-resistant, 20% were rifampicin-resistant and 19% were MDR. Drug-resistant isolates had the following frequency of mutations in rpoB (48%), katG (14%), inhA (26%), ahpC (26%). Susceptible isolates also had a mutation in ahpC (29%). Conclusions This is the first analysis of mutations associated with MDR of M. tuberculosis in Guadalajara. Commonly reported mutations worldwide were found in rpoB, katG and inhA genes. Substitution C to T in position -15 of the ahpC gene may possibly be a polymorphism. " 2015 Elsevier España, S.L.U.