Yeast species associated with spontaneous fermentation of taberna, a traditional palm wine from the southeast of Mexico

Abstract

Morphological variation of the Y chromosome has been observed in different populations. This variation is mostly related to the heteromorphic Yq12 band, which is composed of a variable block of constitutive heterochromatin. The Yqh+ heteromorphism has a worldwide frequency of 2.85% and is considered clinically innocuous. The aim of this study was to identify the ancestry of the Yqh+ heteromorphism present in individuals from western Mexico. For this purpose, 17 Y-chromosome single nucleotide polymorphisms were analysed by multiplex polymerase chain reaction and SNaPshot assays. In 28 Yqh+ males, only five haplogroups were observed; with a haplogroup diversity of 0.4841 0.1094, which was less than that observed in a study of unselected Mexican mestizo population. Differences were specifically conferred by the high frequencies of haplogroups R1b1 and P*(xQ,R), and by the absence of the Amerindian haplogroup Q (Q*(xQ1a3a) plus Q1a3a) from the Yqh+ group. This study suggests a post-1492 incorporation for Yqh+ chromosomes into the Mexican northwestern population. " 2012 Indian Academy of Sciences.",,,,,,"10.1007/s12041-012-0187-x",,,"http://hdl.handle.net/20.500.12104/45769","http://www.scopus.com/inward/record.url?eid=2-s2.0-84873127583&partnerID=40&md5=35cdaaffb645681d5e5b8c7324363b5f",,,,,,"3",,"Journal of Genetics",,"297

302",,"91",,"Scopus

WOS",,,,,,"ancestry; haplogroups; Y-chromosome single nucleotide polymorphisms; Yq12 heteromorphism",,,,,,"Y-SNP haplogroups related to the Yqh+ heteromorphism in the Mexican northwestern population",,"Article" "47542","123456789/35008",,"Santiago-Urbina, J.A., Laboratorio 321, Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, México, D. F. 04510, Mexico; Arias-García, J.A., Laboratorio de Biotecnología, Centro universitario de ciencias biológicas y agropecuarias, Universidad de Guadalajara, A.P.139-1 Zapopan Jalisco, Guadalajara, 45101, México; Ruiz-Terán, F., Laboratorio 321, Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, México, D. F. 04510, Mexico",,"Santiago-Urbina, J.A.

Arias-García, J.A.

Ruiz-Teran, F.",,"2014",,"The aim of this study was to isolate and identify the yeasts present in taberna, a traditional palm wine from Mexico, which is produced by natural fermentation of the palm sap obtained from Acrocomia aculeata. A total of 450 yeast isolates were obtained from 45 taberna samples collected over 15 days of tapping at the end of fed-batch fermentation (12 hours of feeding and fermentation of the sap in the canoe) from three coyol palm trees. The yeast isolates were identified using restriction pattern analysis of the internal transcribed spacer region 5.8S-ITS and by sequence analysis of the D1/D2 divergent domain of the 26S rRNA gene. Nine different yeast species were identified in the taberna samples tested, namely, Saccharomyces cerevisiae, Hanseniaspora guilliermondii, Candida tropicalis, Candida intermedia, Kazachstania unispora, Kazachstania exigua, Meyerozyma guilliermondii, Pichia kudriavzevii (Issatchenkia orientalis) and Pichia kluyveri. The non-Saccharomyces yeasts H. guilliermondii and C. tropicalis were detected in samples from all three palm trees, while S. cerevisiae was detected in samples from only two of the palm trees. The frequency and distribution of the yeast species were different in the samples of each palm tree, which indicated that the inoculum in the palm sap may be deposited randomly by different vectors. This study is the first to characterize the yeast population associated with the palm wine named taberna. " 2014 Springer-Verlag Berlin Heidelberg and the University of Milan.