Modeling SDF-1-induced mobilization in leukemia cell lines

Abstract

As part of the process of globalization, business models and approaches are being developed that aim to both create profits for the participating companies and reduce poverty. These models include BoP (Base of the Pyramid), Inclusive Business and Business with the Base of the Pyramid. This chapter will focus on the base of the pyramid approach as it is the most widely known and is being adopted by important agencies such as the Inter-American Development Bank as its focus. This chapter will aim to identify and look critically at the underlying assumptions and beliefs behind this model. In so doing, it will further examine the ethical considerations that are at the root of this approach. Having identified key ethical considerations, a number of interviews will be conducted with people involved in these initiatives in three different Latin American countries focusing on how they have dealt with these ethical issues in practice. The data collected from these real cases will provide input for analysis. Using the ethical framework constructed in the chapter, three case studies will be offered by conducting interviews with people working in this field in different Latin American countries. " 2012, IGI Global.",,,,,,"10.4018/978-1-61350-332-4.ch016",,,"http://hdl.handle.net/20.500.12104/38961","http://www.scopus.com/inward/record.url?eid=2-s2.0-84898152946&partnerID=40&md5=8cd00501e470298e09ba1d5fecdc7d97",,,,,,,,"Ethical Models and Applications of Globalization: Cultural, Socio-Political and Economic Perspectives",,"238

261",,,,"Scopus",,,,,,,,,,,,"A critical examination of the assumptions, beliefs and ethical considerations that underlie business models of global poverty reduction",,"Book Chapter" "44669","123456789/35008",,"Zepeda-Moreno, A., Department of Medicine V, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany; Saffrich, R., Department of Medicine V, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany; Walenda, T., Stem Cell Biology and Cellular Engineering, Helmholtz Institute for Biomedical Engineering, Cell Biology, RWTH Aachen Medical School, Aachen, Germany; Hoang, V.T., Department of Medicine V, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany; Wuchter, P., Department of Medicine V, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany; Sánchez-Enríquez, S., Department of Molecular Biology and Genomics, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico; Corona-Rivera, A., Department of Molecular Biology and Genomics, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico; Wagner, W., Stem Cell Biology and Cellular Engineering, Helmholtz Institute for Biomedical Engineering, Cell Biology, RWTH Aachen Medical School, Aachen, Germany; Ho, A.D., Department of Medicine V, University Hospital Heidelberg, Heidelberg University, Heidelberg, Germany",,"Zepeda-Moreno, A.

Saffrich, R.

Walenda, T.

Hoang, V.T.

Wuchter, P.

Sanchez-Enriquez, S.

Corona-Rivera, A.

Wagner, W.

Ho, A.D.",,"2012",,"The stromal cell-derived factor 1 (SDF-1) is essential for circulation, homing, and retention of hematopoietic stem cells in the bone marrow. Present evidence indicates that this factor might play an important role in leukemia cells as well. The aim of this study is to present a model of SDF-1-induced mobilization using leukemia cell lines. CXCR4 expression was compared in Kasumi-1, Jurkat, HL-60, KG-1a, and K562 cells by flow cytometry and Western blot. Migration was analyzed with Transwell assays, and adhesive cell-cell interaction was quantified with a standardized adhesion assay and flow cytometry. CXCR4 was expressed by all leukemic cell lines analyzed, although surface expression of this receptor was found in Kasumi-1 and Jurkat cells only. Correspondingly, SDF-1? effects on migration and cell-cell adhesion were observed in Kasumi-1 and Jurkat cells only, and this could be blocked by AMD3100 in a reversible manner. We have provided evidence that SDF-1? acts as a chemotactic and chemokinetic agent. In addition, surface expression of integrin-?2, activated leukocyte cell adhesion molecule and N-cadherin decreased after stimulation with SDF-1?. SDF-1? affects cell-cell adhesion and migration only in leukemia cells on which the CXCR4 receptor is present on the surface. An SDF-1 gradient is not necessarily required to induce migration, as chemokinesis can also occur. Upon stimulation with SDF-1, CXCR4 promotes modifications on the surface pattern of adhesion molecules, which have an influence on adhesion and migration. " 2012 ISEH - Society for Hematology and Stem Cells.