Article
Genetic diversity and relationships among wild and cultivated Stenocereus queretaroensis populations in western Mexico
Fecha
2013Autor
Cabrera-Diaz, E.
Barbosa-Cardenas, C.M.
Perez-MontanO, J.A.
Gonzalez-Aguilar, D.
Pacheco-Gallardo, C.
Barba, J.
Institución
Resumen
The occurrence, serotype diversity, and antimicrobial resistance of Salmonella bacteria in commercial ground beef at retail establishments were investigated. Salmonella was isolated from 135 (56.7%) of 238 ground beef samples collected at the same number of butcher's shops located in three municipalities of Jalisco State, Mexico, during an 11-month period. The isolation frequency differed by municipality (P < 0.05) and was higher (P < 0.05) during the warm season (68.5%) than during the cold season (43.2%). Overall, 25 serotypes and 8 serogroups were identified among 135 Salmonella isolates; predominant were Salmonella group B (9.6%), Salmonella Anatum (8.9%), Salmonella Agona (6.7%), Salmonella Infantis (6.7%), and Salmonella Typhimurium (5.9%). All Salmonella isolates were tested for susceptibility to 11 antimicrobial drugs of human and veterinary use. Resistance to tetracycline was the most commonly observed (40.7%), followed by resistance to streptomycin (35.6%), trimethoprim-sulfamethoxazole (20.7%), and nalidixic acid (19.3%). Thirty-seven Salmonella isolates (27.4%) were multidrug resistant, and the majority corresponded to Salmonella Group B, Salmonella Anatum, and Salmonella Typhimurium. Three Salmonella isolates were resistant to seven different antimicrobials. The frequency of Salmonella in ground beef samples (56.7%) was higher than that observed in our previous investigation on beef carcasses (15.4%) at small abattoirs in the same region of Mexico. This may be a result of increasing contamination at these two points of the raw-beef production chain or may be an effect of the grinding process that facilitates a more-homogeneous pathogen distribution in the product. Poor hygiene, temperature abuse, and practices allowing cross-contamination during ground beef fabrication at these retail establishments increase the consumer's exposure to Salmonella. " International Association for Food Protection.",,,,,,"10.4315/0362-028X.JFP-13-109",,,"http://hdl.handle.net/20.500.12104/43296","http://www.scopus.com/inward/record.url?eid=2-s2.0-84889640220&partnerID=40&md5=d6dd007b81ad3eabf6580db7089786aa http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=medl&AN=24290673",,,,,,"12",,"Journal of Food Protection",,"2004 2010",,"76",,"Scopus MEDLINE WOS",,,,"Index Medicus;Abattoirs;Animals;Anti-Bacterial Agents/pd [Pharmacology];Cattle;Colony Count, Microbial;Drug Resistance, Multiple, Bacterial;Food Contamination/an [Analysis];Food Microbiology;Humans;Meat Products/mi [Microbiology];Mexico/ep [Epidemiology];Microbial Sensitivity Tests;Prevalence;Salmonella/cl [Classification];Salmonella/de [Drug Effects];Salmonella/ip [Isolation & Purification];Salmonella;Salmonella Food Poisoning/ep [Epidemiology];Salmonella Food Poisoning/pc [Prevention & Control];Serotyping",,,,,,,,"Occurrence, serotype diversity, and antimicrobial resistance of salmonella in ground beef at retail stores in jalisco state, mexico",,"Article"
"43475","123456789/35008",,"Ruan-Tejeda, I., Laboratorio de Zoología, Departamento de Ecología y Recursos Naturales, Centro Universitario de la Costa Sur, Universidad de Guadalajara, Av. Independencia Nacional 151, Autlán, Jalisco CP 48900, Mexico; Santerre, A., Laboratorio de Biomarcadores Moleculares y Genética Molecular, Departamento de Biología Celular y Molecular, Universidad de Guadalajara, Centro Universitario de Ciencias Biológicas y Agropecuarias, Km. 15.5 Carretera Guadalajara-Nogales, Predio Las Agujas, Zapopan, Jalisco, Mexico; Huerta-Martínez, F.M., Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Km. 15.5 Carretera Guadalajara-Nogales, Predio Las Agujas, Zapopan, Jalisco, Mexico; Iñiguez-Dávalos, L.I., Laboratorio de Zoología, Departamento de Ecología y Recursos Naturales, Centro Universitario de la Costa Sur, Universidad de Guadalajara, Av. Independencia Nacional 151, Autlán, Jalisco CP 48900, Mexico; Castro-Félix, P., Laboratorio de Biomarcadores Moleculares y Genética Molecular, Departamento de Biología Celular y Molecular, Universidad de Guadalajara, Centro Universitario de Ciencias Biológicas y Agropecuarias, Km. 15.5 Carretera Guadalajara-Nogales, Predio Las Agujas, Zapopan, Jalisco, Mexico",,"Ruan-Tejeda, I. Santerre, A. Huerta-Martinez, F.M. Iniguez-Davalos, L.I. Castro-Felix, P.",,"2014",,"Stenocereus queretaroensis is an endemic, chiropterophilous, columnar cactus of economic importance in Mexico. To investigate the effect of artificial selection on genetic diversity, inter-simple sequence repeat (ISSR) markers were used to estimate the genetic variation of wild, orchard, and backyard populations of S. queretaroensis from two regions in western Mexico. Six primers were used to generate 62 bands, of which 39 were polymorphic (62.9%). The total genetic diversity was similar in the wild (HT=0.296) and orchard (HT=0.291) groups, and slightly lower in the backyard group (HT=0.281). Wild populations (FST=0.13) were less differentiated than backyard (FST=0.17) and orchard (FST=0.21) populations. The wild and backyard groups were genetically closer (0.015) than the wild and orchard (0.018) groups. A Mantel test revealed a positive correlation between genetic and geographic distances (r=0.433, p=0.002). In conclusion, gene flow and the prevailing management system have efficiently maintained genetic diversity and facilitated inter-population differentiation in S. queretaroensis. " 2014 Elsevier Ltd.