Polyhydroxybutyrate production by Saccharophagus degradans using raw starch as carbon source

Abstract

The originally wild species of the Meccus complex are important vectors of Chagas disease in Mexico. In West Mexico, Meccus longipennis plays an important epidemiological role. To understand the genetic structure of the domestic and wild populations of this species, a preliminary study with five polymorphic microsatellite loci was conducted. The population genetics analysis showed high structuring between peridomestic biotopes, with breeding subunits detected in a single peridomestic structure. In the wild environment, two genetic patterns were observed according to the biotope, possible breeding subunits in large rocky formations and a larger panmictic unit in agropastoral areas, suggesting considerable dispersal of bugs in this biotope. Moreover, the discovery of two foci of wild populations at the edge of Guadalajara city raises the question of new urban areas where the phenomenon of bug incursions into households could constitute a risk of transmission of Chagas disease. " 2011 Elsevier B.V.",,,,,,"10.1016/j.meegid.2011.11.003",,,"http://hdl.handle.net/20.500.12104/43741","http://www.scopus.com/inward/record.url?eid=2-s2.0-84857566578&partnerID=40&md5=961f2b8a13a552149dfba2c8ccb384fa",,,,,,"2",,"Infection, Genetics and Evolution",,"254

262",,"12",,"Scopus

WOS",,,,,,"Chagas disease; Mexico; Microsatellite; Population genetics; Triatomine",,,,,,"Population genetic structure of Meccus longipennis (Hemiptera, Reduviidae, Triatominae), vector of Chagas disease in West Mexico",,"Article" "45482","123456789/35008",,"González-García, Y., Department of Wood, Cellulose and Paper, CUCEI, University of Guadalajara, carretera Guadalajara-Nogales, Zapopan, Jalisco, Mexico; Rosales, M.A., Department of Chemical Engineering, CUCEI, University of Guadalajara, Blvd. Marcelino García-Barragan, Guadalajara, Jalisco, Mexico; González-Reynoso, O., Department of Chemical Engineering, CUCEI, University of Guadalajara, Blvd. Marcelino García-Barragan, Guadalajara, Jalisco, Mexico; Sanjuan-Dueñas, R., Department of Wood, Cellulose and Paper, CUCEI, University of Guadalajara, carretera Guadalajara-Nogales, Zapopan, Jalisco, Mexico; Córdova, J., Department of Chemical Engineering, CUCEI, University of Guadalajara, Blvd. Marcelino García-Barragan, Guadalajara, Jalisco, Mexico",,"Gonzalez-García, Y.

Rosales, M.A.

Gonzalez-Reynoso, O.

Sanjuan-Duenas, R.

Cordova, J.",,"2011",,"Biosynthesis of poly(3-hydroxybutyrate) (PHB) from raw starch as the carbon source by the polysaccharide-digesting bacteria Saccharophagus degradans was investigated in a fed-batch culture. The production and properties of the PHB synthesized from starch were compared to those obtained using glucose as carbon source. In fed-batch cultures, S. degradans accumulated 21.35 and 17.46% of PHB, using glucose or starch as carbon source, respectively. The physical properties of the biopolymer produced from each carbon source were similar between them. Molecular mass, melting temperature and heat of fusion were 54.23kDa, 165.61°C and 59.59J/g, respectively, using glucose; and 57.07kDa, 174.31°C and 67.66J/g, respectively, using starch. This is the first work describing the capability of S. degradans to utilize raw starch as the sole carbon source for the production of PHB. " 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.