Conference Paper
Optical tweezers experiments for fibroblast cell growth stimulation
Fecha
2014Autor
Zamora-Bustillos, R.
Rivera-Reyes, R.
Aguilar, M.B.
Michel-Morfin, E.
Landa-Jaime, V.
Falcon, A.
Heimer, E.P.
Institución
Resumen
Marine snails of the genus Conus (?500 species) are tropical predators that produce venoms for capturing prey, defense and competitive interactions. These venoms contain 50-200 different peptides ("conotoxins") that generally comprise 7-40 amino acid residues (including 0-5 disulfide bridges), and that frequently contain diverse posttranslational modifications, some of which have been demonstrated to be important for folding, stability, and biological activity. Most conotoxins affect voltage- and ligand-gated ion channels, G protein-coupled receptors, and neurotransmitter transporters, generally with high affinity and specificity. Due to these features, several conotoxins are used as molecular tools, diagnostic agents, medicines, and models for drug design. Based on the signal sequence of their precursors, conotoxins have been classified into genetic superfamilies, whereas their molecular targets allow them to be classified into pharmacological families. The objective of this work was to identify and analyze partial cDNAs encoding precursors of conotoxins belonging to I superfamily from three vermivorous species of the Mexican Pacific coast: C. brunneus, C. nux and C. princeps. The precursors identified contain diverse numbers of amino acid residues (C. brunneus, 65 or 71; C. nux, 70; C. princeps, 72 or 73), and all include a highly conserved signal peptide, a C-terminal propeptide, and a mature toxin. All the latter have one of the typical Cys frameworks of the I-conotoxins (C-C-CC-CC-C-C). The prepropeptides belong to the I2-superfamily, and encode eight different hydrophilic and acidic mature toxins, rather similar among them, and some of which have similarity with I2-conotoxins targeting voltage- and voltage-and-calcium-gated potassium channels. " 2014 Elsevier Inc.",,,,,,"10.1016/j.peptides.2014.01.018",,,"http://hdl.handle.net/20.500.12104/42045","http://www.scopus.com/inward/record.url?eid=2-s2.0-84899434478&partnerID=40&md5=4c21f79220be5586bf912a171dce37a1 http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=medl&AN=24486530",,,,,,,,"Peptides",,"22 29",,"53",,"Scopus WOS MEDLINE",,,,"Index Medicus;Amino Acid Sequence;Animals;Conotoxins/ch [Chemistry];Conotoxins/ge [Genetics];Conus Snail/ge [Genetics];DNA, Complementary;Mexico;Molecular Sequence Data;Potassium Channels, Voltage-Gated/ch [Chemistry];Potassium Channels, Voltage-Gated/ge [Genetics];Reverse Transcriptase Polymerase Chain Reaction/mt [Methods];Sequence Homology, Amino Acid;Snails/ge [Genetics]",,"cDNA cloning; Conotoxin precursor; Conus brunneus; Conus nux; Conus princeps; I-conotoxin",,,,,,"Identification, by RT-PCR, of eight novel I2-conotoxins from the worm-hunting cone snails Conus brunneus, Conus nux, and Conus princeps from the eastern Pacific (Mexico)",,"Article"
"43830","123456789/35008",,"Jiménez-Domínguez, B., Urban Studies Center, University of Guadalajara, Mexico; López Aguilar, R.M., Department of Applied Psychology, University of Guadalajara, Mexico",,"Jimenez-Dominguez, B. Lopez Aguilar, R.M.",,"2002",,"As part of the international project City-Identity-Sustainability, a study was carried out in two popular neighborhoods in the city of Guadalajara. One of the neighborhoods, Analco, was traditional and well consolidated; the other, Los Colorines, was new and in the process of consolidation. An adapted survey was applied to evaluate identity, sustainability, and demographic data. Results suggest that the relationship between greater or lesser consolidation and practices of sustainability is mediated mainly by factors of identity and collective satisfaction with the place.",,,,,,"10.1177/0013916502034001007",,,"http://hdl.handle.net/20.500.12104/42051","http://www.scopus.com/inward/record.url?eid=2-s2.0-0036139603&partnerID=40&md5=47961261efeae3b2e48cd700191b5081",,,,,,"1",,"Environment and Behavior",,"97 110",,"34",,"Scopus WOS",,,,,,,,,,,,"Identity and sustainability in two neighborhoods of Guadalajara, Mexico",,"Article"
"45167","123456789/35008",,"Avila, R., Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, A.P. 1-1010, Santiago-de Querétaro 76000, Mexico; Medina-Villalobos, N., Centro Universitario de Los Lagos, Universidad de Guadalajara, Paseos de la Montaña 1144, Lagos-de Moreno, Jalisco 47463, Mexico; Tamariz, E., Instituto de Ciencias de la Salud, Departamento de Biomedicina, Universidad Veracruzana, Luis Castelazo Ayala s/n, 91190, Xalapa, Veracruz, Mexico; Chiu, R., Centro Universitario de Los Lagos, Universidad de Guadalajara, Paseos de la Montaña 1144, Lagos-de Moreno, Jalisco 47463, Mexico; Lopez-Marín, L.M., Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, A.P. 1-1010, Santiago-de Querétaro 76000, Mexico; Acosta, A., Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, A.P. 1-1010, Santiago-de Querétaro 76000, Mexico; Castaño, V., Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, A.P. 1-1010, Santiago-de Querétaro 76000, Mexico",,"Avila, R. Medina-Villalobos, N. Tamariz, E. Chiu, R. Lopez-Marin, L.M. Acosta, A. Castano, V.",,"2014",,"Optical tweezers constitute an increasingly used tool for the study of biomechanical properties of cells. Here we report experiments for the projection induction of NIH3T3 fibroblast cells, using a single-trap optical tweezers. The system is based on a 1064-nm, 50mW infrared gaussian laser beam, a 100x microscope objective with 1.25 numerical aperture and a temperature-controlled warming plate to maintain cell viability. Eighteen cells were exposed to the focussed laser beam in different cell zones and another 18 cells were observed without laser stimulation as a control population. The results show that the probability of lamelipodia growth increases on exposed cells by a factor 1.5. " 2014 SPIE.