Journal Article
Antimycobacterial and HIV-1 Reverse Transcriptase Activity of Julianaceae and Clusiaceae Plant Species from Mexico.
Fecha
2015Registro en:
1741-427X; 1741-427X
10.1155/2015/183036
Autor
Gomez-Cansino R
Espitia-Pinzon CI
Campos-Lara MG
Guzman-Gutierrez SL
Segura-Salinas E
Echeverria-Valencia G
Torras-Claveria L
Cuevas-Figueroa XM
Reyes-Chilpa R
Institución
Resumen
The extracts of 14 Julianaceae and 5 Clusiaceae species growing in Mexico were tested in vitro (50microg/mL) against Mycobacterium tuberculosis H37Rv and HIV reverse transcriptase (HIV-RT). The Julianaceae bark and leaf extracts inhibited M. tuberculosis (>84.67%) and HIV-RT (58.3% and >67.6%), respectively. The IC50 values for six selected extracts and their cytotoxicity (50microg/mL) to human macrophages were then determined. Amphipterygium glaucum, A. molle, and A. simplicifolium fairly inhibited M. tuberculosis with IC50 of 1.87-2.35microg/mL; but their IC50 against HIV-RT was 59.25-97.83microg/mL. Calophyllum brasiliense, Vismia baccifera, and Vismia mexicana effect on M. tuberculosis was noteworthy (IC50 3.02-3.64microg/mL) and also inhibited RT-HIV (IC50 26.24-35.17microg/mL). These 6 extracts (50microg/mL) presented low toxicity to macrophages (<23.8%). The HPLC profiles of A. glaucum, A. molle, and A. simplicifolium indicated that their antimycobacterial activity cannot be related to masticadienonic, 3alpha, or 3beta-hydromasticadienonic acids, suggesting that other compounds may be responsible for the observed activity or this might be a synergy result. The anti-HIV-RT and antimycobacterial activities induced by C. brasiliense can be attributed to the content of calanolides A, B, as well as soulatrolide.