Identificación de un elemento regulador común para la expresión de los genes KiSS1 y REN en placentas de mujeres con preeclampsia-eclampsia comparado con placentas de mujeres con embarazo normo-evolutivo

Abstract

A B S T R A C T: The aim of the present work was to look at differences in the placental tissue expression of KiSS-1 and REN genes from preeclamptic and healthy pregnant women, that could account for a possible synergistic function for both genes in the pathogenesis of preeclampsia. Study design: This case–control study involved 27 preeclamptic women and 27 normoevolutive pregnant women. cDNA was obtained from placental tissue to carry out qPCR for both KiSS-1 and REN genes in order to compare mRNA expression levels in the studied groups. Statistical analysis showed expression differences that correlate with clinical and/or biochemical variables. Results: Higher expression for KiSS-1 in PEE vs. control woman (p = 0.001) was observed, whereas no difference was observed for REN expression (p = 0.300) when all the subjects were included. However, REN expression was significant higher when the samples were stratified according to preeclampsia severity. For 18 mild preeclamptic patients the p-value was p = 0.001 compared to their controls, while for the remaining nine with severe preeclampsia the expression became significant (p = 0.001). Conclusion: Our results suggest that the high KiSS-1 expression seen in preeclamptic patients is in accordance with its role as an inhibitor of trophoblast invasiveness and maintained until the end of gestation. On the other hand, aggressive therapeutic management and/or severity status of patients have a direct effect on placental REN expression levels, masking the natural high expression of this gene on preeclamptic placental tissue. Therefore it was not possible to establish a real concordant expression profile for KiSS-1 and REN genes.

La preeclampsia es la principal complicación de los trastornos específicos del embarazo. La identificación de marcadores para su desarrollo se ha convertido en un área importante de la investigación biomédica. El objetivo fue identificar algún elemento de regulación transcripcional común para la expresión de los genes KiSS-1 y REN en tejido placentero de mujeres con preeclampsia-eclampsia, comparándolo con mujeres con embarazo normo-evolutivo.