Artículo de revista
Dlg Is required for short-term memory and interacts with NMDAR in the drosophila brain
Fecha
2022Registro en:
Int. J. Mol. Sci. 2022, 23, 9187
10.3390/ijms23169187
Autor
Bertin, Francisca
Moya Alvarado, Guillermo Adrián
Quiroz Manríquez, Eduardo
Ibacache, Andrés
Köhler Solís, Andrés
Oliva, Carlos
Sierralta Jara, Jimena Alejandra
Institución
Resumen
The vertebrates’ scaffold proteins of the Dlg-MAGUK family are involved in the recruitment,
clustering, and anchoring of glutamate receptors to the postsynaptic density, particularly the NMDA
subtype glutamate-receptors (NRs), necessary for long-term memory and LTP. In Drosophila, the only
gene of the subfamily generates two main products, dlgA, broadly expressed, and dlgS97, restricted
to the nervous system. In the Drosophila brain, NRs are expressed in the adult brain and are involved
in memory, however, the role of Dlg in these processes and its relationship with NRs has been
scarcely explored. Here, we show that the dlg mutants display defects in short-term memory in the
olfactory associative-learning paradigm. These defects are dependent on the presence of DlgS97 in
the Mushroom Body (MB) synapses. Moreover, Dlg is immunoprecipitated with NRs in the adult
brain. Dlg is also expressed in the larval neuromuscular junction (NMJ) pre and post-synaptically and
is important for development and synaptic function, however, NR is absent in this synapse. Despite
that, we found changes in the short-term plasticity paradigms in dlg mutant larval NMJ. Together our
results show that larval NMJ and the adult brain relies on Dlg for short-term memory/plasticity, but
the mechanisms differ in the two types of synapses.