Properties and structures of three- and four-component microemulsions

Abstract

The production and characterization of polyhydroxyalkanoic acids (PHAs) from glucose in Pseudomonas aeruginosa ATCC 9027 is described. We determined that the synthesis of PHAs was not due to a complete lack of nitrogen source, as previously reported for other microorganisms. The synthesis of PHAs was observed during exponential growth and it depended on the carbon/nitrogen ratio in the culture. More significantly, the specific PHA accumulation rate in this phase was higher than that observed in the storage phase. This phenomenon was a consequence of higher extracellular production rates of gluconate and 2-ketogluconate detected during the storage phase. Therefore, the production of those acids decreased the synthesis of PHAs in P. aeruginosa. The maximum percentage of PHA accumulation obtained was 10.8% of the cell dry matter when all the glucose was consumed. The monomer composition of this PHA consisted only of saturated 3-hydroxy fatty acids (octanoic, decanoic, and dodecanoic acids) as shown by gas chromatography - mass spectroscopy and nuclear magnetic resonance analyses, where 3-hydroxydecanoic acid was the main component because of the high affinity of its PhaC synthase for this monomer. The physical properties of this PHA were determined by differential scanning calorimetry and gel permeation chromatography. " 2007 NRC.",,,,,,"10.1139/W07-023",,,"http://hdl.handle.net/20.500.12104/43907","http://www.scopus.com/inward/record.url?eid=2-s2.0-35748966451&partnerID=40&md5=0524b86dcd43cda02eaad04615a6d795",,,,,,"7",,"Canadian Journal of Microbiology",,"840

851",,"53",,"Scopus

WOS",,,,,,"Extracellular oxidation; PHAs; Pseudomonas aeruginosa",,,,,,"Production and characterization of polyhydroxyalkanoates in Pseudomonas aeruginosa ATCC 9027 from glucose, an unrelated carbon source",,"Article" "45696","123456789/35008",,"Prieto-Miranda, S.E., Educación e Investigación en Salud, Hospital General Regional Núm. 46 Lázaro Cárdenas, Guadalajara, Jalisco, Mexico, Departamento de Terapia, Intensiva Del Nuevo Hospital Civil de Guadalajara, Jalisco, Mexico; Monjaraz-Guzmán, E.G., Hospital General Regional Núm. 46 Lázaro Cárdenas, IMSS, Mexico; Díaz-Pérez, H.M., Universidad de Guadalajara, Mexico; González-Bojórquez, E., Universidad de Guadalajara, Mexico; Jiménez-Bernardino, C.A., Hospital de Especialidades Del Centro Médico Nacional de Occidente, Guadalajara, Jal, Mexico; Esparza-Pérez, R.I., Hospital de Especialidades Del Centro Médico Nacional de Occidente, Guadalajara, Jal, Mexico",,"Prieto-Miranda, S.E.

Monjaraz-Guzman, E.G.

Diaz-Perez, H.M.

Gonzalez-Bojorquez, E.

Jimenez-Bernardino, C.A.

Esparza-Perez, R.I.",,"2012",,"In the last 15 years medical schools and medical colleges of the American union have been working hard in the study and implementation of a professionalism more in line with the society, with doctors in training, both undergraduate and graduate, as physicians currently in practice, motivated probably by high levels of dissatisfaction with their society, assuming this one a questioning and demanding posture. In our environment there is no change in the traditional teaching in this area with doctors in training and physicians who already practice their profession, despite showing the same indicators of dissatisfaction. The responsibility for training better doctors with professionalism, must be of the health authorities and institutions that form the health staff, and from the doctors in training, remembering that our profession has a contract with the society which we serve and sooner or later will claim these changes.",,,,,,,,,"http://hdl.handle.net/20.500.12104/43917","http://www.scopus.com/inward/record.url?eid=2-s2.0-84869072946&partnerID=40&md5=300971c02b8fa6c9d28d35163d479dcb",,,,,,"5",,"Medicina Interna de Mexico",,"496

503",,"28",,"Scopus",,,,,,"Ethics; Medical; Principles; Professionalism",,,,,,"Professionalism in medicine [Profesionalismo en medicina]",,"Note" "45709","123456789/35008",,"Puig, J.E., Facultad de Ciencias Químicas, Universidad de Guadalajara, Guadalajara, Jal 44430, Mexico; Rodríguez-Siordia, I., Facultad de Ciencias Químicas, Universidad de Guadalajara, Guadalajara, Jal 44430, Mexico; Rangel-Zamudio, L.I., Departmento de Química, Cinvestav Mexico, D.F. 07000, Mexico; Billman, J.F., Department of Chemical Engineering, BF-10, University of Washington, Seattle, WA 98195, United States; Kaler, E.W., Department of Chemical Engineering, BF-10, University of Washington, Seattle, WA 98195, United States",,"Puig, J.E.

Rodriguez-Siordia, I.

Rangel-Zamudio, L.I.

Billman, J.F.

Kaler, E.W.",,"1988",,"We report phase behavior, small-angle X-ray scattering (SAXS), fluorescence spectroscopy, conductimetry, and differential scanning calorimetry of didodecyldimethylammonium bromide (DDAB)/cyclohexane/water and DDAB/decane/water microemulsions with and without NaBr. We demonstrate that the hydrocarbon tails of the surfactant are fluid in microemulsions at room temperature. The observed phase behavior exhibits a complex dependence upon salt concentration, yet microemulsion structure as probed by SAXS and conductimetry is not altered by the addition of salt. Pyrene fluorescence is used to probe the internal interfacial layers, and evidence for the compression of the surfactant tails with decreasing curvature is obtained. " 1988 American Chemical Society.