Article
Carboxyl ester hydrolases production and growth of a halophilic archaeon, Halobacterium sp. NRC-1
Fecha
2010Autor
Gomora-Arrati, P.
Gonzalez-Arenas, A.
Balandran-Ruiz, M.A.
Mendoza-Magana, M.L.
Gonzalez-Flores, O.
Camacho-Arroyo, I.
Institución
Resumen
Several changes in brain function, including learning and memory, have been reported during pregnancy but the molecular mechanisms involved in these changes are unknown. Due to the fundamental role of glial cells in brain activity, we analyzed the content of glial fibrillary acidic protein (GFAP) in the hippocampus, frontal cortex, preoptic area, hypothalamus and cerebellum of the rat on days 2, 14, 18, and 21 of pregnancy and on day 2 of lactation by Western blot. A differential expression pattern of GFAP was found in the brain during pregnancy and the beginning of lactation. GFAP content was increased in the hippocampus throughout pregnancy, whereas a decrease was observed in cerebellum. GFAP content was increased in the frontal cortex and hypothalamus on days 14 and 18, respectively, with a decrease in the following days of pregnancy in both regions. In preoptic area a decrease in GFAP content was observed on day 14 with an increase on days 18 and 21. In the frontal cortex and cerebellum, GFAP content was increased on day 2 of lactation, while it was maintained as on day 21 of pregnancy in the other regions. Our data suggest a differential expression pattern of GFAP in the rat brain during pregnancy and the beginning of lactation that should be associated with changes in brain function during these reproductive stages. " 2010 Elsevier Ireland Ltd.",,,,,,"10.1016/j.neulet.2010.08.052",,,"http://hdl.handle.net/20.500.12104/39967","http://www.scopus.com/inward/record.url?eid=2-s2.0-77956923208&partnerID=40&md5=3adc02eab934c27eaea0f071148cadbe",,,,,,"3",,"Neuroscience Letters",,"197 200",,"484",,"Scopus WOS",,,,,,"Cytoskeleton; GFAP; Glial Cells; Pregnancy; Sex steroid hormones",,,,,,"Changes in the content of GFAP in the rat brain during pregnancy and the beginning of lactation",,"Article"
"41721","123456789/35008",,"Domínguez, M.G., CIBO-IMSS, CUCS-U de G, Mexico; Vásquez, A.I., CIBO-IMSS, CUCS-U de G, Mexico; Troyo, R., División de Disciplinas Básicas, CUCS-U de G, Guadalajara Jal., Mexico; Ortiz-Aranda, M., CIBO-IMSS, CUCS-U de G, Mexico; Padilla, J.R., CIBO-IMSS, CUCS-U de G, Mexico; Hernández-Zaragoza, G., CIBO-IMSS, CUCS-U de G, Mexico; Rivas, F., CIBO-IMSS, CUCS-U de G, Mexico; Rivera, H., CIBO-IMSS, CUCS-U de G, Mexico, Centro de Investigación Biomédica de Occidente-IMSS, Ap. Postal 1-3838, Guadalajara, Jal., Mexico",,"Dominguez, M.G. Vasquez, A.I. Troyo, R. Ortiz-Aranda, M. Padilla, J.R. Hernandez-Zaragoza, G. Rivas, F. Rivera, H.",,"2006",,"We characterized two Y-ring microchromosomes (MC) found in an azoospermic patient with Turner stigmata (case A) and a male infant with hypospadias (case B). The karyotypes, as assessed by banding, FISH, and STRs/STSs analyses, were 46,X,r(Y).ish r( Y)(p11.3q11.222)(SRY+,DYZ3+) and 46,X,+r(Y)/45,X.ish r(Y)(p11.2q11.2)(Xp/Yp-,SRY+,DYZ3+) respectively. In both cases, we evaluated the association of each MC with the centromere of the nearest and second nearest chromosomes in G-banded metaphases by means of measuring the intervening distance according to two criteria: ?1 time or ?3 times the size of the MC in each metaphase. The case A's MC was associated 84 times in 98 cells according to the latter or less strict criterion and two times in 98 cells according to the strict criterion; the corresponding values for case B were 84 and two in 95 cells respectively. The centromeric association appears to be related to centromeric attraction mediated by heterochromatin or centromere-specific proteins, the replication time, and the Rabl orientation.",,,,,,,,,"http://hdl.handle.net/20.500.12104/39942","http://www.scopus.com/inward/record.url?eid=2-s2.0-33847253833&partnerID=40&md5=7e8168207d98c4fb93e3a300f7e36ddb",,,,,,"4",,"Genetic Counseling",,"413 419",,"17",,"Scopus WOS",,,,,,"Centromere association; Microchromosomes; Rabl orientation",,,,,,"Centromeric association of a microchromosome y in two male patients",,"Article"
"41690","123456789/35008",,"Camacho, R.M., Centro de Investigación y Asistencia, en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Guadalajara, Jalisco, Mexico; Mateos-Díaz, J.C., Centro de Investigación y Asistencia, en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Guadalajara, Jalisco, Mexico; Diaz-Montaño, D.M., Centro de Investigación y Asistencia, en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Guadalajara, Jalisco, Mexico; González-Reynoso, O., Departamento de Ing. Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico; Córdova, J., Departamento de Ing. Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico",,"Camacho, R.M. Mateos-Diaz, J.C. Diaz-Montano, D.M. Gonzalez-Reynoso, O. Cordova, J.",,"2009",,"The capability of Halobacterium sp. NRC-1 to synthesize carboxyl ester hydrolases was investigated, and the effect of physicochemical conditions on the growth rate and production of esterases was evaluated. The haloarchaeon synthesized a carboxyl ester hydrolase, confirming the genomic prediction. This enzymatic activity was intracellularly produced as a growth-associated metabolite. Esterase activity was assayed using different p-nitrophenyl-esters and triacyl-glycerides, which showed a preference for hydrolyzing tributyrin. The archaeal growth rate and esterase production were significantly influenced by the pH and the NaCl concentration. An interaction effect between temperature and NaCl was also seen. The maximal growth rate and esterase production found for Halobacterium sp. NRC-1 were 0.136 h-1 (at 4.2 M NaCl, pH 6 and 44°C) and 1.64 U/l (at 4.6 M NaCl, pH 6 and 30°C), respectively. Furthermore, the effects of NaCl concentration, pH and temperature on enzyme activity were studied. Two maximal esterase activities were elucidated from the intracellular crude extract when it was incubated at different NaCl concentrations (1 M and 5 M) and at different pHs (6 and 7.5). This is the first report that shows experimentally the synthesis of carboxyl ester hydrolases by Halobacterium sp. NRC-1. This enzyme was found to be extremely halophilic (5 M NaCl) and thermophilic (80°C), making it very interesting for future investigations in non-aqueous biocatalysis. " Springer 2009.