Article
Decreased Corticotropin-Releasing F actor-Like Immunoreactivity in Rat Intermediate and Posterior Pituitary after Stalk Section
Author
Saavedra, J.M
Rougeot, Catherine
Culman, Juruj
Israel, Anita
Níwa, Masami
Tonon, Marie Christine
Vaudry, Hubert
Dray, Fernand
Institutions
Abstract
We determined the corticotropin-releasing factor(CRF)-like immunoreactivity by radioimmunoassay in intermediate
and posterior pituitary lobes of stalk-sectioned and sham-operated control rats. The antigenic determinant read by
the CRF antibody used was contained within the region of amino acids 26 (Gln) to 37 (Lcu) ofthe molecule. Intermediate
and posterior lobes of control rats contained similar amounts of CRF (591 + 78 and 487 ! 34 pg/mg protein, respectively).
The section ofthe pituitary stalk p¡oduced a marked dec¡ease in CRFlike immunoreactivity in both structures. The
CRF content on the intermediate lobe after stalk section was 62 t l7 pg/ng ptotein, a decrease of 90%, and that of the
posterior lobe was 90 + 13 pg,hg protein, an 83% decrease. Our results suggest that most ofthe CRF-like immunoreactivity
in the ¡at intermediate and posterior pituitary lobes is contained in nerve fibers ofbrain origin and support the hypothesis
of a ¡ole of CRF in the release of intermediate and posterior lobe peptides.
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