Decreased Corticotropin-Releasing F actor-Like Immunoreactivity in Rat Intermediate and Posterior Pituitary after Stalk Section

dc.creatorSaavedra, J.M
dc.creatorRougeot, Catherine
dc.creatorCulman, Juruj
dc.creatorIsrael, Anita
dc.creatorNíwa, Masami
dc.creatorTonon, Marie Christine
dc.creatorVaudry, Hubert
dc.creatorDray, Fernand
dc.date2011-06-20T19:46:23Z
dc.date2011-06-20T19:46:23Z
dc.date1984-02-01
dc.date.accessioned2022-10-28T00:47:27Z
dc.date.available2022-10-28T00:47:27Z
dc.identifierhttp://hdl.handle.net/10872/167
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4935061
dc.descriptionWe determined the corticotropin-releasing factor(CRF)-like immunoreactivity by radioimmunoassay in intermediate and posterior pituitary lobes of stalk-sectioned and sham-operated control rats. The antigenic determinant read by the CRF antibody used was contained within the region of amino acids 26 (Gln) to 37 (Lcu) ofthe molecule. Intermediate and posterior lobes of control rats contained similar amounts of CRF (591 + 78 and 487 ! 34 pg/mg protein, respectively). The section ofthe pituitary stalk p¡oduced a marked dec¡ease in CRFlike immunoreactivity in both structures. The CRF content on the intermediate lobe after stalk section was 62 t l7 pg/ng ptotein, a decrease of 90%, and that of the posterior lobe was 90 + 13 pg,hg protein, an 83% decrease. Our results suggest that most ofthe CRF-like immunoreactivity in the ¡at intermediate and posterior pituitary lobes is contained in nerve fibers ofbrain origin and support the hypothesis of a ¡ole of CRF in the release of intermediate and posterior lobe peptides.
dc.languageen_US
dc.publisherNeuroendocrinology
dc.subjectCorticotropin-releasing factor
dc.subjectPituitary gland
dc.subjectIntermediate pituitary
dc.subjectPosterior pituitary
dc.subjectPituitary stalk section
dc.titleDecreased Corticotropin-Releasing F actor-Like Immunoreactivity in Rat Intermediate and Posterior Pituitary after Stalk Section
dc.typeArticle


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