artículo científico
Equine antibodies to Bothrops asper myotoxin II: isolation from polyvalent antivenom and neutralizing ability.
Fecha
1990Registro en:
0041-0101
10.1016/0041-0101(90)90075-I
2349580
Autor
Lomonte, Bruno
Gutiérrez, José María
Carmona Morera, Elena
Rovira Rodríguez, María Elena
Institución
Resumen
Equine antibodies to
B. riper myotoxin II were isolated from polyvalent antivenom by affinity
chromatography. Purified antibodies were among the most acidic serum
immunoglobulins, migrating between the ß- and aZ-globulin regions by zone
electrophoresis . This might be related to the fact that myotoxin II is a very
basic antigen. At an antibody/toxin molar ratio of two or higher, myotoxic
effect of myotoxin II was neutralized completely . Antibodies also neutralized
myotoxic activity of crude venom in mice, reducing the effect by about 75%
when a proportion of 16 mg antibodies/mg venom was tested . Despite the lack
of phospholipase AZ activity of myotoxin II, antibodies were able to neutralize
this enzymatic activity of myotoxin I, a previously described isoform. This
finding is in agreement with the notion that myotoxin II is a
phospholipase AZ-analog devoid of this enzyme activity.