Tesis
Análise da microbiota dominante de três pontos amostrais do solo da savana amazônica com potencial na produção de bioativos
Fecha
10-10-2012Registro en:
Autor
SANTOS, Jerônimo Dias dos
Institución
Resumen
The state of Amapá has the most conserved and least known biodiversity of Brazil, especially
the microbial biodiversity of its soil, including fungi, yeasts and bacteria, such as
actinomycetes, which are known for their potential for the synthesis of bioactive compounds
such as antimicrobial agents, and for this reason, many of these compounds are primarily used
by the pharmaceutical industry. Therefore, this research project held a screening of
microorganism on the soil samples amazonian savanna of the State of Amapá, and isolated
microorganisms capable of synthesizing bioactive compounds with antibiosis potential. For
that, colonies of microorganisms were isolated from soil samples on selective medium, and
these were subcultured in specific media for growth of soil microorganisms. The isolated
microorganisms were characterized by macroscopic and microscopic observation of colonies
characteristics and the aerial mycelium by methods such as the microculture, and these were
also classified as bacteria, fungi and yeasts, as well as the description of the morphology of
microorganisms and their groupings by Gram staining. The total of 40 microorganisms were
isolated, and all of these were tested for antibiosis plate against standardized microorganisms
for such methodology by the method of crossed trace (cris-cross). From the isolated
microorganisms, 6 are Gram-positive bacterias, 11 Gram-negative, 7 filamentous fungi and 16
are yeasts, from these last, 12 isolated microorganism have showed significant antimicrobial
activity in antibiosis test plate