Effect of the precipitating agent on the immobilization of Aspergillus oryzae ?-galactosidase by cross-linking and aggregation

Abstract

Enzymatic synthesis of lactulose with β galactosidase is still a scarcely explored field and most reported yields are low For this reason, new strategies are required for lactulose synthesis allowing an increase in selectivity, yield and productivity  Enzyme immobilization usually produces a significant increase in operational stability and allows catalyst recovery, being in many cases the key for making enzymatic processes technologically and economically viable Cross linked aggregates of precipitated β galactosidase CLAGs belong to a new generation of biocatalysts not requiring an inert matrix, offering many economic and environmental benefits in the context of industrial biocatalysis, namely, a very high mass specific activity, simple production and low cost, being active and stable in non conventional (non aqueous) media, and having acceptable mechanical properties  The objective of this work is the evaluation of different conditions of immobilization of Aspergillus oryzae β galactosidase AOG by aggregation and crosslinking To this purpose, different types and concentrations of precipitating agents PA time of cross linking and concentration of the cross linking agent ( were evaluated in the manufacture of CLAGs