Artículos de revistas
Deciphering Additional Roles for the EF-Tu,l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli
Fecha
2020-08Registro en:
Torres, A. N., Chamorro-Veloso, N., Costa, P., Cádiz, L., Del Canto, F., Venegas, S. A., ... & Vidal, R. M. (2020). Deciphering additional roles for the EF-Tu, L-asparaginase II and OmpT proteins of Shiga toxin-producing Escherichia coli. Microorganisms, 8(8), 1184.
2076-2607
WOS: 000568106600001
PMID: 32759661
10.3390/microorganisms8081184
Autor
Torres, Alexia N.
Chamorro-Veloso, Nayaret [Univ Mayor, Biome Therapeut, Chile]
Costa, Priscila
Cádiz, Leandro
Del Canto, Felipe
Venegas, Sebastián A.
López Nitsche, Mercedes
Coloma-Rivero, Roberto F.
Montero, David A.
Vidal, Roberto M.
Institución
Resumen
Shiga toxin-producingEscherichia coli(STEC) causes outbreaks and sporadic cases of gastroenteritis. STEC O157:H7 is the most clinically relevant serotype in the world. The major virulence determinants of STEC O157:H7 are the Shiga toxins and the locus of enterocyte effacement. However, several accessory virulence factors, mainly outer membrane proteins (OMPs) that interact with the host cells may contribute to the virulence of this pathogen. Previously, the elongation factor thermo unstable (EF-Tu),l-asparaginase II and OmpT proteins were identified as antigens in OMP extracts of STEC. The known subcellular location of EF-Tu andl-asparaginase II are the cytoplasm and periplasm, respectively. Therefore, we investigate whether these two proteins may localize on the surface of STEC and, if so, what roles they have at this site. On the other hand, the OmpT protein, a well characterized protease, has been described as participating in the adhesion of extraintestinal pathogenicE. colistrains. Thus, we investigate whether OmpT has this role in STEC. Our results show that the EF-Tu andl-asparaginase II are secreted by O157:H7 and may also localize on the surface of this bacterium. EF-Tu was identified in outer membrane vesicles (OMVs), suggesting it as a possible export mechanism for this protein. Notably, we found thatl-asparaginase II secreted by O157:H7 inhibits T-lymphocyte proliferation, but the role of EF-Tu at the surface of this bacterium remains to be elucidated. In the case of OmpT, we show its participation in the adhesion of O157:H7 to human epithelial cells. Thus, this study extends the knowledge of the pathogenic mechanisms of STEC.