Artículos de revistas
Interleukin-35 inhibits alveolar bone resorption by modulating the Th17/Treg imbalance during periodontitis
Fecha
2020-04Registro en:
Cafferata, E. A., Terraza‐Aguirre, C., Barrera, R., Faúndez, N., González, N., Rojas, C., ... & González, F. E. (2020). Interleukin‐35 inhibits alveolar bone resorption by modulating the Th17/Treg imbalance during periodontitis. Journal of Clinical Periodontology, 47(6), 676-688.
0303-6979
1600-051X
ID de PubMed: 32160331
Número WOS: WOS:000523200200001
10.1111/jcpe.13282
Autor
Cafferata, Emilio A.
Terraza-Aguirre, Claudia
Barrera, Romina
Faúndez, Nicolás
González, Nicolás
Rojas, Carolina
Melgar-Rodríguez, Samanta
Hernández, Marcela
Carvajal, Paola
Cortez, Cristian [Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Chile]
González, Fermin E.
Covarrubias, Cristian
Vernal, Rolando
Institución
Resumen
Aim T lymphocytes play a central role during the pathogenesis of periodontitis, and the imbalance between the pathogenic T-helper type 17 (Th17) and protective T-regulatory (Treg) lymphocytes determines the tooth-supporting alveolar bone resorption. Interleukin (IL)-35 is a novel anti-inflammatory cytokine with therapeutic properties in diseases whose pathogenesis is associated with the Th17/Treg imbalance; however, its role during periodontitis has not been established yet. This study aimed to elucidate whether IL-35 inhibits the alveolar bone resorption during periodontitis by modulating the Th17/Treg imbalance. Materials and Methods Mice with ligature-induced periodontitis were treated with locally or systemically administrated IL-35. As controls, periodontitis-affected mice without IL-35 treatment and non-ligated mice were used. Alveolar bone resorption was measured by micro-computed tomography and scanning electron microscopy. The Th17/Treg pattern of the immune response was analysed by qPCR, ELISA, and flow cytometry. Results IL-35 inhibited alveolar bone resorption in periodontitis mice. Besides, IL-35 induced less detection of Th17 lymphocytes and production of Th17-related cytokines, together with higher detection of Treg lymphocytes and production of Treg-related cytokines in periodontitis-affected tissues. Conclusion IL-35 is beneficial in the regulation of periodontitis; particularly, IL-35 inhibited alveolar bone resorption and this inhibition was closely associated with modulation of the periodontal Th17/Treg imbalance.