info:eu-repo/semantics/article
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
Fecha
2005-09Registro en:
Cámara, María Silvia; Mastandrea, Carlos Raúl; Goicoechea, Hector Casimiro; Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods; Elsevier Science; Journal Of Biochemical And Biophysical Methods; 64; 9-2005; 153-166
0165-022X
CONICET Digital
CONICET
Autor
Cámara, María Silvia
Mastandrea, Carlos Raúl
Goicoechea, Hector Casimiro
Resumen
In the present report, CBZ is determined on real patients samples by using a procedure completely assisted by chemometric tools. Firstly, a response surface methodology based on a mixture design was applied in order to select the best conditions in the extraction step. Finally, multivariate calibration PLS-1 was applied to second derivative UV spectra eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) method comparison with HPLC and FPIA methods applied on real patient samples. The results obtained show the potentiality of the presently studied methodology for the following of patients treated with this anticonvulsivant.